TABLE 2.
Oligonucleotides used in this study
| Name | Sequence (5′–3′) | Target |
|---|---|---|
| P1740 | ATTGGAAGTGGATAACGGTACCGGTTCCGAGGCTC | pKOR1-mcs |
| P1741 | ATTGGATTGGAAGTACGGGCCCGAGCTTAAGACTGG | pKOR1-mcs |
| P1742 | ATGTCTATTTAAAACTTTTCTTCATATTGTAAACTCC | argR upstream |
| P1743 | TACTTCCAATCCAATGCGTGAATCATATGACAACCAAGG | argR upstream |
| P1744 | TATGAAGAAAAGTTTTAAATAGACATGTATCAAATGAATA | argR downstream |
| P1745 | TTATCCACTTCCAATGAAACAAGTTCATCGCCGCCTTC | argR downstream |
| P3356 | TTGATATCGAATTCGGAGGCCACAAGGGTTTGGAGGAGTAGCAA | putA |
| P3357 | TTATTATCCGGAGGTGTAGCCCAGACTCCTAAATTATTGTTTATGTCTTGCT | putA |
| P4135 | GATTACGCCGAGCAATAACAACGACGCCTT | |
| P4136 | CGGCCAGGTTTCAGAACTGCCAATATAACATTTAGGAGC | |
| P4137 | TATTGCTCGGCGTAATCATGGTCATAGCTGTT | |
| P4138 | TCTGAAACCTGGCCGTCGTTTTACAACGT | |
| CRE-F | ACCCTTACATCAAAAACAATCAAAAATAATTATGTATAATTACACTA | |
| CRE-R | TTTCAAGAATATTTTTTTCATGCTAAACTTATTGTAAACACAAGGG | |
| PrD-F | GCTATGACCATGATTACGCCGAAGGGTTTTATCCATTACCACG | |
| PrD-R | AAATTCTTTAATAGTGCCATCATATCTCCCCTTTTCATCA | |
| Vec-F | GGTAATGGATAAAACCCTTCGGCGTAATCATGGTCATAG | |
| Vec-R | TGATGAAAAGGGGAGATATGATGGCACTATTAAAGAATTTTTTTATCG | |
| P3358 | TACTCCTCCAAACCCTTGTGGCCTCCGAATTCGATATCAAGCT | pYJ335 |
| P3359 | ATAATTTAGGAGTCTGGGCTACACCTCCGGATAATAAATATATATAAACGTATATAGATT | pYJ335 |
| P3418 | TTGATATCGAATTCGGAGGCGTATAAATATAAAGGAGGGAGACCGATGAATTCAAT | argDCJB |
| P3419 | TTATTATCCGGAGGTGTAGCGCGCAATGGATAATCGAACTGGAAT | argDCJB |
| P3416 | GATTATCCATTGCGCGCTACACCTCCGGATAATAAATATATATAAACGTATATAGATTTC | pYJ335 |
| P3417 | TCCCTCCTTTATATTTATACGCCTCCGAATTCGATATCAAGCTTATT | pYJ335 |
| P5173 | AAATAAGCTTGATCAAAAACTAAAGGGATGTGACGTTAATG | proC |
| P5174 | CATTATCTCATATTATTGGTCTTCTATATTAGAAAGTTCAATACTACGGTCG | proC |
| P5175 | CTTTAGTTTTTGATCAAGCTTATTTTAATTATACTCTATCAATGATAGAGTGTCAAT | pYJ335 |
| P5176 | AGAAGACCAATAATATGAGATAATGCCGACTGTACTTTTTACAGT | pYJ335 |
| P3348 | AGCCTTAAAGACGATCCGGGCGACGCCTTTCGCATCATTTTCA | putA promoter |
| P3349 | GTTCACCACCTTTTCCCTATGTGCCATTGCTACTCCTCCAAAC | putA promoter |
| P3350 | TGGAGGAGTAGCAATGGCACATAGGGAAAAGGTGGTGAACTACT | pOS1-P2640-lacZ |
| P3351 | AAATGATGCGAAAGGCGTCGCCCGGATCGTCTTTAAGGCT | pOS1-P2640-lacZ |
| P3352 | AGCCTTAAAGACGATCCGGGGGCATCAATTTAATGAAGGGTTTTATCCATTACC | rocD promoter |
| P3353 | GTTCACCACCTTTTCCCTATGTGCTCCGTAATGATTTGTTAACTCAATAATTTTTTCAG | rocD promoter |
| P3354 | AACAAATCATTACGGAGCACATAGGGAAAAGGTGGTGAACTACT | pOS1-P2640-lacZ |
| P3355 | CCCTTCATTAAATTGATGCCCCCGGATCGTCTTTAAGGCT | pOS1-P2640-lacZ |
| P3551 | AAATGATGCGAAAGGCGTCGCCCGGATCGTCTTTAAGGCT | pOS1-PputA-lacZ |
| Δ1 | TGTAGATTTCTATTTATAGTATTATTGTTGTCCAT | Cis-element deletion |
| Δ2 | GTCCATATTATTATATATAAATGAAATCAACATCAATAATAGTG | Cis-element deletion |
| Δ3 | CAACATCAATAATAGTGTAATTATACATAATTATTTTTGATTG | Cis-element deletion |
| Δ4 | CATAATTATTTTTGATTGTTTTTGATGAAAACGC | Cis-element deletion |
| Δ5 | TTGATGAAAACGCTTTCTCGAATATTTTTTTC | Cis-element deletion |
| Δ6 | GCTTTCTCGAATATTTTTTTCATGCTAAAC | Cis-element deletion |
| P4105 | CATGGTATATCTCCTTCTTAAAGTTAAAC | pET28a |
| P4106 | CACCACCACCACCACCACTGAGATC | pET28a |
| P4910 | CTTTAAGAAGGAGATATACCATGGCACTATTAAAGAATTTTTTTATCGGATTATC | putA |
| P4911 | GTGGTGGTGGTGGTGTTTACGGCATAATTTTTTAATTGTACTTAAACCTAAC | putA |
| P142 | AAACTGATTTTTAGTAAACAGTTGACGATATTC | Inverse PCR |
| P143 | TTTATGGTACCATTTCATTTTCCTGCTTTTTC | Inverse PCR |
| 16S-F | TATAGATGGATCCGCGCT | qRT-PCR |
| 16S-R | CTTTCGCCCATTGCGGAAG | qRT-PCR |
| putA-F | GTTAGCAGAAGAAATCGCAAATG | qRT-PCR |
| putA-R | GTGGGCGTTCTGCTAATCTT | qRT-PCR |
| arcB-F | GGTGGCAACATATTAATCACAG | qRT-PCR |
| arcB-R | CCATTGATACCCAAACGTCAG | qRT-PCR |
| argG-F | GATCGAACGCATCCTCTGGT | qRT-PCR |
| argG-R | GGGCATGGAGTCGTGAAGAA | qRT-PCR |
| argD-F | TGGGAGCAAGTCGTTCCAGA | qRT-PCR |
| argD-R | CAAGCGCTGCCGTCGATATT | qRT-PCR |
| argF-F | TTGCAGCGCATGATCAAGGT | qRT-PCR |
| argF-R | TTCCACACTGGTACGCCTGAA | qRT-PCR |
| rocF-F | CTAAATCTCTCATACCAATTAGTAC | qRT-PCR |
| rocF-R | TAAGGATCCGGGGGACGCTTATGACAAAGAC | qRT-PCR |
| rocD-F | TAAGGATCCGATATGATGACTAAATCTGAAAAAATT | qRT-PCR |
| rocD-R | ATCTCCAAAATCAACTTTTCTAAATC | qRT-PCR |
| proC-F | TGGCACAAGCTATATTTACAGG | qRT-PCR |
| proC-R | TTTGATGCGTGTTGCTAGAG | qRT-PCR |
| P3183 | CGTCCCAATTTTTCTAAAGGATGG | Transduction |
| P3184 | CACTCAAGCAACTGTTTCTCGTG | Transduction |