TABLE 1.
Genes predicted by TnSeq that were previously shown to be required for swarming motilitya
| Gene | PSb | Categoryc | Annotationd | Reference |
|---|---|---|---|---|
| cheA | 1 | Motility | Chemotaxis, run generator | 1 |
| cheC | 1 | Motility | fla/che operon, polar | 8 |
| cheD | 1 | Motility | fla/che operon, polar | 8 |
| cheW | 1 | Motility | fla/che operon, polar | 8 |
| cheY | 1 | Motility | Chemotaxis, run generator | 1 |
| cwlO | 1 | Envelope | PG hydrolase | 24 |
| degU | 1 | Regulation | Flagellar regulation | 18 |
| flgB | 1 | Motility | Flagellar structure | 82 |
| flgC | 1 | Motility | Flagellar structure | 82 |
| flgD | 1 | Motility | Flagellar structure | 36 |
| flgE | 1 | Motility | Flagellar structure | 36 |
| flgK | 1 | Motility | Flagellar structure | 38 |
| flgL | 1 | Motility | Flagellar structure | 38 |
| flgM | 1 | Motility | Flagellar regulation, polar | 82 |
| flgN | 1 | Motility | Flagellar structure | 83 |
| flhA | 1 | Motility | Flagellar assembly | 82 |
| flhB | 1 | Motility | Flagellar assembly | 82 |
| flhF | 1 | Motility | Flagellar regulation, polar | 13 |
| flhG | 1 | Motility | Flagellar regulation, polar | 13 |
| flhO | 1 | Motility | Flagellar structure | 36 |
| flhP | 1 | Motility | Flagellar structure | 36 |
| fliD | 1 | Motility | Flagellar structure | 84 |
| fliE | 1 | Motility | Flagellar structure | 82 |
| fliF | 1 | Motility | Flagellar structure | 82 |
| fliG | 1 | Motility | Flagellar structure | 82 |
| fliH | 1 | Motility | Flagellar assembly | 82 |
| fliI | 1 | Motility | Flagellar assembly | 82 |
| fliJ | 1 | Motility | Flagellar assembly | 82 |
| fliK | 1 | Motility | Flagellar regulation | 36 |
| fliL | 1 | Motility | Flagellar function, polar | 82 |
| fliM | 1 | Motility | Flagellar structure | 82 |
| fliO | 1 | Motility | Flagellar assembly | 82 |
| fliP | 1 | Motility | Flagellar assembly | 82 |
| fliQ | 1 | Motility | Flagellar assembly | 82 |
| fliR | 1 | Motility | Flagellar assembly | 82 |
| fliS | 1 | Motility | Flagellar assembly | 84 |
| fliY | 1 | Motility | Flagellar structure | 82 |
| gsaB | 1 | Translation | EF-P modification | 45 |
| hag | 1 | Motility | Flagellar structure | 1 |
| motA | 1 | Motility | Flagellar function | 37 |
| motB | 1 | Motility | Flagellar function | 37 |
| pdeH | 1 | Regulation | Flagellar function | 85 |
| pnpA | 1 | Regulation | Flagellar regulation | 19 |
| sigD | 1 | Motility | Flagellar regulation | 86 |
| swrA | 1 | Motility | Flagellar regulation | 8 |
| swrB | 1 | Motility | Flagellar assembly | 8 |
| swrC | 1 | Motility | Surfactin resistance | 8 |
| swrD | 1 | Motility | Flagellar function | 61 |
| ylxF | 1 | Motility | fla/che operon, polar | 82 |
| ymfI | 1 | Translation | EF-P activation | 44 |
| ynbB | 1 | Translation | EF-P modification | 45 |
| cheB | 2 | Motility | fla/che operon, polar | 8 |
| cwlQ | 2 | Envelope | PG hydrolase | 24 |
| minJ | 2 | Division | FtsZ localization factor | 87 |
| yabR | 2 | Unknown | Unknown | 8 |
| clpC | 3 | Regulation | AAA+ protease, unknown | 7 |
| clpX | 3 | Regulation | AAA+ protease, unknown | 7 |
| efp | 3 | Translation | Elongation factor P | 8 |
| rho | 3 | Transcription | Transcriptional terminator | 88 |
Table first sorted by priority score (PS) and second alphabetically by gene name.
Each gene was assigned a priority score (PS) based on data analysis. A score of 3 indicates that the gene passed the criteria of a ratio of 0.1 or lower and a statistical P value of <0.05 and could not be eliminated by manual evaluation of the data. A score of 2 indicates that the gene did not pass the ratio cutoff but was rescued because it appeared to have an insertion differential by visual scanning of the data set. A score of 1 indicates that the gene passed the quantitative criteria and also showed strong insertion differential by visual scanning of the data set.
Category is a general category of function assigned to the presumed or known function.
Annotation is a brief specific description of the known or predicted function of the gene product. “Polar” indicates that deletion of the gene does not impair swarming but transposon insertions within it are known to have polar effects on motility-essential genes downstream.