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. 2022 Jun 7;13:903272. doi: 10.3389/fpls.2022.903272

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Copyright © 2022 Chen, Shen, Chou, Sato and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduced contents of UDP-N-acetylhexosamines (UDP-HexNAc) in salt-treated iU1 seedlings. (A) Reduction in UDP-HexNAc in iU1 seedlings. UDP-HexNAc was analyzed by LC/MS from 14-day-old seedlings grown on MS agar media supplemented with or without NaCl (200 mM) or NaCl + GlcNAc (100 μM). Data represent the means ± SD of four biological replicates. ^*p < 0.05. Student’s t-test. (B) Phenotypic comparison. Seedlings were grown under the same conditions as those shown in (A). (C) Seedlings with greening cotyledons in (B) were quantified. Data represent the means ± SD of at least four biological replicates, each with 60–80 seeds. ^*p < 0.05, Student’s t-test.