TABLE 1.
Difference alignments of probe target sites indicating the discriminatory mismatchesa
| Probe (positionb) and organism | Target |
|---|---|
| EHB412c (412–429) | |
| EHBs | 5′-ACACCCCUAUGGGGCGUA-3′ |
| Hydrogenobacter thermophilus | 5′-.........C....G.C.-3′ |
| Sphingobacterium heparinum | 5′-...G....C....U....-3′ |
| Rhodothermus marinus | 5′-GA.G.U..UC....U...-3′ |
| Rhodothermus obamensis | 5′-GA.G....UC....U...-3′ |
| EHB586 (586–603) | |
| EHB-1 | 5′-GGGCAGCAAGUCGGAUGU-3′ |
| EHB-2 | 5′-.....A............-3′ |
| EHB1451 (1451–1468) | |
| EHB-2 | 5′-AGCCGGAGGGAGAGCGGC-3′ |
| EHB-1 | 5′-UUUG........C.GCCG-3′ |
a
Results are based on analysis of probe checks against at least 13,000 complete and partial sequences (ARB software; see text).
b
E. coli positions (7).
c
No sequences with one or two mismatches were found for probe EHB412.