Figure 2.

Inactivation of the infralimbic (IL) cortex by lidocaine or microinjection of protein synthesis inhibitor has no effect on retrieval or extinction consolidation of recent or remote odor aversion memory. (A, C) Schematic presentation of the experimental protocol for recent (A) and remote (C) COA memory and its extinction. (B, D) Animals’ IL cortex was infused with lidocaine (Lido) or vehicle 15 minutes before the first testing day (T1) to inhibit memory retrieval or with anisomycin (Aniso) or vehicle immediately after T1 to inhibit extinction consolidation. T1 occurred 2 days (recent memory, B) or 28 days (remote memory, D) after conditioning and was followed by 2 additional days of testing (T2, T3). On each testing day, animals were subjected to testing preference for the odorized water. Aversion index was measured as milliliters of water consumption/total fluid consumption (water + banana) × 100, with a maximal score of 100 for strong COA and a score of 50 indicating no COA. As aversion index did not differ between the 2 different vehicle groups, they were merged into a single group (V). Error bars show SEM. No significant differences were found between vehicle, lidocaine, and anisomycin groups.