Table 1.

Measurements of unconjugated 25OHD3 in QC samples after hydrolysis of 25OHD3-S by 3 arylsulfatase enzymes and 25OHD3-G by beta-glucuronidase

	25OHD3 (ng/mL)
Hydrolysis enzyme	QCL (25OHD3-S 2.5 ng/mL)	QCM (25OHD3-S 10 ng/mL)	QCH (25OHD3-S 60 ng/mL)
PVFV (n = 6)	2.52 ± 0.09 (100.8%)	10.82 ± 0.30 (102.8%)	59.2 ± 1.09 (98.7%)
WT (n = 3)	2.56 ± 1.22 (102.4%)	10.65 ± 0.22 (106.5%)	60.1 ± 1.24 (100.2%)
DRN (n = 3)	1.22 ± 0.02 (48.8%)	7.74 ± 0.27 (77.4%)	48.9 ± 1.50 (81.5%)
	QCL (25OHD3-G 0.25 ng/mL)	QCM (25OHD3-G 1 ng/mL)	QCH (25OHD3-G 15 ng/mL)
Beta-glucuronidase (n = 6)	0.253 ± 0.018 (101.2%)	1.10 ± 0.12 (110.0%)	15.1 ± 0.36 (100.7%)

Data expressed as mean ± standard deviation (% conversion).