Table 3.
Serum concentrations of unconjugated, sulfate and glucuronide 25OHD3 and the percentage of 25OHD3 as conjugate forms in samples from men aged 70+ with and without vitamin D supplementation
| 25OHD3 (ng/mL) | 25OHD3-S (ng/mL) | 25OHD3-G (ng/mL) | |||
|---|---|---|---|---|---|
| No hydrolysis | Hydrolysis | % Conjugated | No hydrolysis | No hydrolysis | |
| Nonsupplement N-89 |
23.1 ± 8.0 22.8 (16.6, 30.0) |
44.8 ± 14.3 44.3 (34.1, 56.1) |
48.2 ± 8.4% | 20.2 ± 7.7 20.0 (14.5, 23.6) |
1.86 ± 0.79 1.77 (1.21, 2.48) |
| D3 supplement N-57 |
26.0 ± 8.5 25.1 (19.7 32.3) |
49.0 ± 15.0 48.8 (36.4, 59.4) |
46.5 ± 9.4% | 21.5 ± 8.7 21.9 (14.5, 25.4) |
1.94 ± 1.10 1.86 (1.14, 2.38) |
| D2 supplement N-24 |
12.4 ± 8.8 8.5 (6.55, 18.0) |
24.1 ± 12.4 21.2 (13.9, 34.7) |
51.1 ± 12.7% | 11.0 ± 4.97 9.85 (7.40,14.98) |
1.04 ± 0.64 0.81 (0.51,1.47) |
Data expressed as mean ± standard deviation, median (IQR 25%, 75%). Unconjugated fraction measured without hydrolysis (No hydrolysis) and combined unconjugated and conjugated fractions measured after hydrolysis by PVFV-PaS and beta-glucuronidase (Hydrolysis). 25OHD3-S and 25OHD3-G measurements performed in samples without hydrolysis. The percentage conjugated value is the proportion of 25OHD3 conjugate forms present in samples that was determined after hydrolysis.