Table 4.
Serum concentrations of 3-epi-25OHD3, 24,25(OH)2D3, and 25OHD2 and the percentage of each analyte as conjugate forms in samples from men aged 70+ with and without vitamin D supplementation
| 3-Epi-25OHD3 (ng/mL) | 24,25(OH)2D3 (ng/mL) | |||||
|---|---|---|---|---|---|---|
| No hydrolysis | Hydrolysis | % Conjugated | No hydrolysis | Hydrolysis | % Conjugated | |
| Nonsupplement N-89 |
1.37 ± 0.61 1.30 (0.91, 1.71) |
1.95 ± 0.76 1.89 (1.37, 2.39) |
30.6 ± 7.5% | 2.00 ± 0.97 1.91 (1.20, 2.68) |
5.30 ± 2.16 5.25 (3.50, 6.60) |
62.5 ± 9.0% |
| D3 supplement N-57 |
1.54 ± 0.75 1.43 (0.96, 1.96) |
2.17 ± 0.95 1.98 (1.47, 2.62) |
29.6 ± 9.7% | 2.30 ± 1.06 2.07 (1.59, 2.86) |
5.84 ± 2.42 5.58 (4.02, 7.17) |
59.1 ± 11.0% |
| D2 supplement N-24 |
0.84 ± 0.66 0.60 (0.45, 0.91) |
1.20 ± 0.83 1.02 (0.70, 1.36) |
31.6 ± 7.5% | 0.95 ± 0.65 0.69 (0.51, 1.31) |
2.91 ± 1.61 2.47 (1.78, 4.24) |
66.2 ± 12.6% |
| 25OHD2 (ng/mL) | ||||||
| No hydrolysis | Hydrolysis | % Conjugated | ||||
| Nonsupplement N-89 |
0.60 ± 0.30 0.52 (0.41, 0.75) |
0.84 ± 0.40 0.75 (0.55, 1.03) |
29.2 ± 10.2% | |||
| D3 supplement N-57 |
0.67 ± 0.54 0.54 (0.35, 0.76) |
1.0 ± 0.73 0.76 (0.53, 1.10) |
29.6 ± 10.0% | |||
| D2 supplement N-24 |
16.25 ± 8.35 17.68 (7.47, 19.90) |
22.54 ± 11.59 23.00 (12.06, 27.98) |
27.3 ± 12.5% | |||
Data expressed as mean ± standard deviation, median (IQR 25%, 75%). Unconjugated fraction measured without hydrolysis (No hydrolysis) and then as combined unconjugated and conjugated fractions measured after hydrolysis by PVFV-PaS and beta-glucuronidase (Hydrolysis). The percentage conjugated value is the proportion of conjugate forms present in samples that was determined after hydrolysis.