Figure 5.

YTHDF3 or IGF2BP2 knockdown inhibited the hypoxia/reoxygenation-induced activation of AKT, NF-κB, ERK1/2, and P38 pathways in BEAS-2B cells. BEAS-2B cells were transfected with or without si-NC, si-YTHDF3, or si-IGF2BP2 for 48 h. Transfected or non-transfected cells received the treatment of normoxia or hypoxia/reoxygenation. Hypoxia/reoxygenation conditions: 6 h of exposure to hypoxic conditions (5% CO₂, 94%N₂, 1% O₂) and another 6 h of normoxic (95% air, 5% CO₂) treatment. Protein levels of AKT, p-AKT, p65, p-p65, ERK1/2, p-ERK1/2, p38, and p-p38 were measured by western blot assay.