FIG. 2.

SDS-PAGE (a) and Western immunoblot analysis (b and c) of purified Ba-S, chimeric enzymes, and A2-5a CGT. (a) Three micrograms of Ba-S, Ch1 Amy, and Ch2 Amy and six micrograms of A2-5a CGT were loaded onto an SDS-polyacrylamide gel, electrophoresed, and stained with Coomassie brilliant blue. Lanes: 1, Ba-S; 2, Ch1 Amy; 3, Ch2 Amy; 4, A2-5a CGT; M, molecular size marker. (b and c) After SDS-PAGE of the sample, the protein was transferred to a nitrocellulose membrane. Anti-Ba-L (b) or anti-CGT (c) antibody was used for the primary antibody. Lanes: 1, Ba-S; 2, A2-5a CGT; 3, Ch1 Amy; 4, Ch2 Amy; M, molecular size marker.