TABLE 2.

Experimental disassociation and stringency results for S-S-Thio-0820-a-A-22 and S-G-Acdp-0821-a-A-24

Probea and target group	No. of mismatches	Experimental Td (°C) ± SD	Avg nontarget signal intensity as % of target intensity (range)
S-S-Thio-0820-a-A-22
T. thiooxidans ATCC 19377	0	46.0 ± 0.17
T. thiooxidans DSM 612	0	46.5 ± 0.42
T. thiooxidans ATCC 19703	Unknown	45.9 ± 0.53
T. thiooxidans ATCC 8085	Unknown	43.9 ± 0.47
T. ferrooxidans ATCC 19859	0	46.8 ± 0.12
T. ferrooxidans ATCC 23270	0	45.6 ± 0.12
All target species		45.8 ± 0.59	0.71 (0.25–1.31)
S-G-Acdp-0821-a-A-24
A. angustum ATCC 35903	0	58.6 ± 0.12
A. cryptum ATCC 33463	0	59.0 ± 0.35
A. organovorum ATCC 43141	0	60.7 ± 0.17
A. acidophilum ATCC 27807	0	62.2 ± 0.42	1.47 (0.30–11.12)
Genus Acidiphilium	0	60.1 ± 1.65
A. diazotrophicus ATCC 49037	2	52.1 ± 0.06

^aSmall-subunit rRNA positions (Escherichia coli numbering) are shown.