TABLE 2.
Collated PfIVT and counterscreen data for compounds from MMV’s Pandemic Box demonstrating >50% inhibition in a single-point IVT screen at 30 μMa
| Compound | PfIVT inhibition at 30 μM (%) | IC50 value (μM) |
||
|---|---|---|---|---|
| PfIVT | HsIVT | Counterscreen | ||
| MMV688991 | 98.3 | 0.02 ± 0.007 | 0.04 ± 0.0003 | 0.04 ± 0.008 |
| MMV002459 | 87.3 | 29 ± 0.9 (>30) | 22 ± 0.3 | >30 |
| MMV1578897 | 93.4 | 0.04 ± 0.008 | 0.18 ± 0.001 | 0.4 ± 0.08 |
| MMV1582495 | 89.9 | 0.6 ± 0.07 | 3.1 ± 0.04 | 1.7 ± 0.08 |
| MMV1578578 | 74.6 | 3.7 ± 0.07 | 10 ± 0.12 | 1.3 ± 0.08 |
| MMV1579781 | 72.4 | 8.1 ± 0.09 | >30 | 0.5 ± 0.08 |
| MMV1580839 | 73.3 | 0.8 ± 0.08 | 7.6 ± 0.12 | 8.3 ± 0.08 |
| MMV124656 | 72.6 | 14 ± 0.9 | 17 ± 0.16 | 3.8 ± 0.08 |
| MMV108465 | 62.6 | 17 ± 0.8 | 23 ± 0.11 | 7.6 ± 0.5 |
| MMV247764 | 63.2 | 14 ± 0.7 | 23 ± 0.2 | 3.2 ± 0.06 |
| MMV141011 | 51.8 | >30 | >30 | 10 ± 0.8 |
| MMV003291 | 52.9 | 2 ± 0.07 | >30 | 0.5 ± 0.08 |
| MMV1634391 | 52.4 | 17 ± 0.6 | 1.1 ± 0.01 | >30 |
a
All IC50 values represent the weighted mean ± standard deviation of two technical replicates. Compounds with IC50 values 1 order of magnitude (10-fold) higher for the counterscreen compared to the PfIVT assay are considered viable hits and are shaded.