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Increased FOS resistance is mediated by upregulation of MurAB expression and increases in PEP flux. (A) qPCR was performed to measure changes in MurAB transcript expression. The assay was conducted in biological triplicates. The housekeeping gene was gyrB. (B) PEP concentration was measured through the PEP colorimetric/fluorometric assay kit (Sigma-Aldrich). Experiments were conducted in biological triplicates with technical duplicates.
