. 2022 May 11;66(6):e02333-21. doi: 10.1128/aac.02333-21

TABLE 1.

Whole-genome sequencing data from DAP-evolved HOU503 isolates^a

Isolate	MIC (μg/mL)^a		Mutation(s) in^b:						Additional mutations^b^,^c
Isolate	DAP	FOS	pgsA	cls	gdpD	CDP-AP	murF	Pyruvate carboxylase	Additional mutations^b^,^c
FT1
D1	64	128		H215R and R211L					+4
D2	64	128		H215R and R211L					+4
D3	64	128		H215R and R211L					+4
D4	64	128				−62	S323R		+6
D5	64	128				−62	S323R		+4
D6	64	170.7		A20D					+4
D7	26.7	128	A98D						+3
D8	32	128							+5
D9	16	128		A20D					+2
D10	64	128				−45		Q1073*	+7
D11	32	128		A20D				Q1073*	+6
D12	9.3	128	V85F					Q1073*	+3
D13	32	170.7		R211L					+4
D14	13.3	128				−62			+4
D15	32	213.3		H215R					+3

FT2
D16	32	128	T70P						+2
D17	26.7	128	T70P						+3
D18	13.3	64	T70P						+2
D19	42.7	64		R211Q					+3
D20	32	128		R211Q					+3
D21	32	64		R211Q					+3
D22	>128	128			L93H	−62			+3
D23	>128	128			L93H	−62			+3
D24	128	128			L93H	−62			+4
D25	21.3	128		H215R					+2
D26	16	64		H215R					+2
D27	16	64		H215R					+2
D28	64	128				−62			+3
D29	64	128				−62			+3
D30	64	64				−62			+3

The MICs reported were averaged over three biological replicates. The HOU503 (LiaR^W73C LiaS^T120A) DAP MIC was 3 μg/mL, and the FOS MIC was 128 μg/mL. * indicate a stop codon.

The selected mutations shown do not include those observed in the no-drug control isolates or initial starting ancestor.

The “Additional mutations” column includes occurrences of van operon (vanRSHAXYZ) loss. D5 and D6 retained one van operon copy, while other isolates lost both copies.