. 2022 May 11;66(6):e02333-21. doi: 10.1128/aac.02333-21

TABLE 3.

Whole-genome sequencing data of DF-evolved HOU503 isolates^c

Isolate	MIC (μg/mL)^a		Mutation(s) in^b:								Additional mutations^b^,^c
Isolate	DAP	FOS	cls	pgsA	CDP-AP	MurAB	pyk	Pyruvate carboxylase	murD	efrB	Additional mutations^b^,^c
FT1
DF1	13.3	682.7				Insertion upstream and −73	S201T			P17Q	+4
DF2	10.7	>1,024				−73	P12S				+5
DF3	10.7	>1,024				−73	S201T			P17Q	+4
DF4	26.7	>1,024	A20D			Insertion upstream	I251N				+3
DF5	>32	>1,024			−62	−73	H37L			N56K	+7
DF6	>32	>1,024			−62	−73	H37L				+7
DF7	26.7	>1,024	A20D			−73	R401C				+3
DF8	32	>1,024	A20D			−73	F31S				+3
DF9	>32	682.7	A20D			−73					+5
DF10	13.3	554.7				−73		Q1073*	K161K		+6
DF11	10.7	469.3				−73		Q1073*	K161K		+5
DF12	5.3	1,024				−73		Q1073*			+7
DF13	>32	512			−62	−73 and G204R					+7
DF14	32	256			−62	−73 and −60					+8
DF15	>32	314.3			−62	Insertion upstream and −73					+7

FT2
DF16	>32	>1,024			−62	Insertion upstream	P330S				+5
DF17	>32	>1,024			−62	Insertion upstream	P330S				+4
DF18	>32	>1,024			−62	Insertion upstream	P330S				+6
DF19	8	>1,024			−62	−73	S246F				+4
DF20	10.7	>1,024			−62	−73	S246F				+4
DF21	16	>1,024			−62	−73	S246F				+4
DF22	16	341.3		A63T		Insertion upstream and −73		Insertion upstream			+3
DF23	10.7	>1,024		A63T		−73					+5
DF24	16	256		A63T		−73		Insertion upstream			+3
DF25	16	1,024	A20D			−73					+5
DF26	16	256	A20D			Insertion upstream and −73					+6
DF27	13.3	1,024	A20D			−73					+5
DF28	8	1,024				−73	A28P				+5
DF29	16	768				−73					+6
DF30	8	256				−73	A28P				+4

The MICs reported were averaged over three biological replicates. The HOU503 (LiaR^W73C LiaS^T120A) DAP MIC was 3 μg/mL, and the FOS MIC was 128 μg/mL. * indicate a stop codon.

The selected mutations shown do not include those observed in the no-drug control isolates or initial starting ancestor.

The “Additional mutations” column includes occurrences of van operon (vanRSHAXYZ) loss. DF1 and DF17 retained one van operon copy, while other isolates lost both copies.