TABLE 1.
Phenotypic and genotypic characteristics of T. indotineae isolates used in this study
| T. indotineae sp. and isolate no.a | Amino acid substitution in SQLE | ITC MIC80 (μg/mL) | VRC MIC80 (μg/mL) | Fold expression of TinCYP51B (mean ± SD)b | TinCYP51B copy no. within genome |
|---|---|---|---|---|---|
| TIMM20114 (UKJ1676/17; IFM 67092) | Ala448Thr | 0.06 | 0.015 | 1 | 1 |
| TIMM20115 (UKJ1700/17; IFM 67093) | Phe397Leu | 0.06 | 0.03 | 1.1 ± 0.4 | 1 |
| TIMM20116 (UKJ1708/17; IFM 67094) | Ala448Thr | 1.0 | 1.0 | 34.0 ± 5.3 | 5 |
| TIMM20117 (200087/18; IFM 67095) | Ala448Thr | 0.5 | 0.5 | 9.6 ± 0.7 | 1 |
| TIMM20118 (UKJ1687/17; IFM 67096) | Phe397Leu | 0.5 | 1.0 | 35.0 ± 12.1 | 7 |
| TIMM20119 (200123/18; IFM 67097) | Phe397Leu | 1.0 | 1.0 | 68.5 ± 25.3 | 5 |
a
All strains were from a previously published resistance study in India, with the numbering in bold (7). They were then preserved in the culture collection of Teikyo University Institute of Medical Mycology (TIMM) and Medical Mycology Research Center, Chiba University (IFM), through the National Bio-Resource Project, Japan (http://www.nbrp.jp/).
b
Results represent expression levels from three independent real-time PCR experiments. Expression levels of TinCYP51B genes were indicated as relative fold changes compared to the CT mean of the data from TIMM20114.