TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pAg1 | Streamlined version of the binary vector pBIN19 containing sequences necessary for replication in E. coli and Agrobacterium tumefaciens (oriV and trfA), E. coli neomycin phosphotransferase gene (nptII), and the transferable DNA (T-DNA) region, with a multiple cloning site within the T-DNA region | 49 |
| pAg1-hph2 | The hph cassette (the promoter sequence of Aspergillus nidulans tryptophan C [trpC] gene [PtrpC] [GenBank accession no. X02390], E. coli hygromycin B phosphotransferase gene [hph], the termination sequence of Aspergillus fumigatus cgrA gene [TcgrA] [GenBank accession no. EAL84894]) | This study |
| pAg1-nptII | The nptII cassette (PtrpC, nptII, TcgrA) | This study |
| pAg1-TinCyp51B/OE | The nptII cassette, the upstream region of T. indotineae translation elongation factor 1-α (TinTef1 α) gene (Ptef1α) (GenBank accession no. OK513035), T. indotineae CYP51B (TinCYP51B) cDNA, TcgrA | This study |
| pAg1-TinCyp51B/T | The 5′ UTR of TinCYP51B gene (2.52 kb) (GenBank accession no. OK539858), the nptII cassette, the 3′ UTR of TinCYP51B gene (2.51 kb) | This study |
| pAg1-wTmeCyp51B/C | The fragment TmeCYP51B1 (the 5′ UTR and wild-type ORFa of TmeCYP51B gene) (3.59 kb), the hph cassette (PtrpC, hph, TcgrA), the 3′ UTR of TinCYP51B gene (2.51 kb) | This study |
| pAg1-mTmeCyp51B/C | The fragment TmeCYP51B2 (the 5′ UTR and mutated ORF of TmeCYP51B gene having a point mutation leading to the Gly443Glu substitution) (3.59 kb), the hph cassette (PtrpC, hph, TcgrA), the 3′ UTR of TinCYP51B gene (2.51 kb) | This study |
| pAg1-TinMDR3/T | The 5′ UTR of TinMDR3 gene (2.60 kb) (GenBank accession no. OK539858), the hph cassette, the 3′ UTR of TinMDR3 gene (2.51 kb) | This study |
| pSilent-1 | The hph cassette (PtrpC, hph, the terminator sequence of A. nidulans trpC gene [TtrpC]), PtrpC, the intron 2 of Magnaporthe oryzae cutinase gene (cut1) (GenBank accession no. X61500), TtrpC | 27 |
| pSilent1-TinCyp51B | The hph cassette (PtrpC, hph, TtrpC), Ptef1 α, the internal sense TinCYP51B cDNA fragment (0.26 kb), the intron 2 of M. oryzae cut1 gene, the internal antisense TinCYP51 B cDNA fragment (0.26 kb), TtrpC | This study |
a
ORF, open reading frame.