TABLE 4.
Susceptibilities to ITC and VRC of T. indotineae transformants overexpressing the TinCYP51B cDNA and corresponding expression levels
| T. indotineae sp. and strain | ITC MIC80 (μg/mL) | VRC MIC80 (μg/mL) | Fold expression of TinCYP51B (mean ± SD)a |
|---|---|---|---|
| TIMM20114 (parent) | 0.06 | 0.015 | 1 |
| Ag38S-1 | 0.25 | 0.06 | 4.3 ± 0.6 |
| Ag38S-7 | 0.125 | 0.03 | 9.3 ± 1.4 |
| Ag38S-10 | 0.125 | 0.03 | 9.9 ± 1.3 |
| Pr38S-22 | 0.25 | 0.06 | 11.5 ± 5.5 |
| Pr38S-23 | 0.25 | 0.06 | 9.1 ± 0.8 |
| Pr38S-30 | 0.5 | 0.125 | 30.0 ± 1.8 |
| Pr38S-31 | 0.5 | 0.125 | 19.9 ± 3.1 |
| Pr38S-37 | 0.5 | 0.06 | 18.1 ± 1.6 |
a
Results represent expression levels from three independent real-time PCR experiments. Expression levels of TinCYP51B genes were indicated as relative fold changes compared to the CT mean of the data from TIMM20114.