TABLE 1.
Susceptibility of clinical Candida spp. to antifungals and nitroxolinea
| Azoles |
Nitroxoline |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Species | Collection ID | AMB | AFG | FLC | VRC | POS | MIC | IZD RPMI | IZD CLSI | IZD MH |
| (mg/L) | (mg/L) | (mg/L) | (mg/L) | (mg/L) | (mg/L) | (mm) | (mm) | (mm) | ||
| C. glabrata (N = 18) | 14-52 16-167 14-64 17-181 19-568 20-372 19-34 18-320 16-205 16-244 18-370 20-215 15-130 16-49 19-294 19-718 18-431 20-753 |
0.25 0.25 0.5 0.5 0.5 0.25 0.5 0.125 0.5 0.5 0.5 0.5 0.25 0.5 0.25 0.25 0.5 0.25 |
2 2 0.06 0.06 ≤0.016 ≤0.016 4 4 2 2 2 2 0.5 0.03 ≤0.016 ≤0.016 0.03 0.03 |
>64 >64 >64 >64 64 >64 4 16 8 4 4 8 0.5 8 8 4 4 2 |
4 8 4 8 1 2 0.125 0.25 0.25 0.06 0.125 0.5 ≤0.016 0.125 0.5 0.125 0.06 0.06 |
>8 >8 4 >8 2 >8 0.5 1 0.5 0.25 0.5 2 0.06 0.5 2 0.125 2 0.125 |
0.25 1 1 2 2 1 1 2 2 2 1 1 2 4 4 4 2 1 |
33 34 35 36 33 31 31 35 30 32 30 31 32 32 32 29 35 32 |
33 27 28 27 28 22 27 26 22 25 25 25 22 24 28 22 20 26 |
28 31 30 31 27 27 27 26 25 27 24 27 26 21 27 23 19 25 |
| C. albicans (N = 8) | 16-166 17-280 20-175 16-238 20-668 18-435 19-51 19-742 |
0.25 0.25 1 0.5 0.125 0.5 0.25 0.125 |
0.5 0.004 ≤0.016 0.016 ≤0.002 0.03 ≤0.016 ≤0.016 |
>64 >64 >64 16 16 0.25 0.25 0.25 |
>8 >8 >8 0.06 0.06 ≤0.016 0.03 0.03 |
>8 >8 >8 0.06 <0.016 0.03 ≤0.016 ≤0.016 |
0.5 0.5 0.5 1 2 1 1 0.5 |
32 31 32 33 27 33 32 35 |
30 29 25 29 25 29 29 30 |
30 28 28 30 23 28 28 27 |
| C. parapsilosis (N = 7) | 20-389 20-548 19-669 19-84 18-227 19-726 20-294 |
0.25 0.25 0.5 0.5 0.5 0.25 0.5 |
1 1 1 1 2 2 1 |
>64 >64 64 32 1 1 0.5 |
4 4 1 1 0.03 0.03 ≤0.016 |
0.03 ≤0.016 0.06 0.03 ≤0.016 ≤0.016 ≤0.016 |
1 0.5 0.25 0.5 2 0.5 0.25 |
33 35 32 35 29 35 40 |
30 28 30 31 24 25 30 |
28 31 27 31 25 25 28 |
| C. auris (N = 5) | 19-731 20-170 20-742 20-39 20-87 |
2 0.5 1 1 1 |
1 0.5 4 0.125 0.125 |
>64 >64 8 16 16 |
>8 2 0.03 0.125 0.125 |
2 ≤0.016 ≤0.016 ≤0.016 ≤0.016 |
1 1 0.5 0.5 1 |
33 35 34 39 38 |
27 27 25 29 30 |
30 29 29 33 33 |
| C. tropicalis (N = 4) | 19-42 17-182 19-318 16-7 |
0.5 0.5 0.5 0.25 |
0.25 0.125 1 0.06 |
>64 >64 0.5 0.5 |
>8 >8 0.125 0.06 |
>8 >8 0.03 0.03 |
2 2 1 1 |
30 32 32 37 |
23 25 31 28 |
30 30 33 36 |
| C. krusei (N = 2) | 17-243 20-694 |
1 0.5 |
0.125 0.03 |
64 32 |
1 0.25 |
0.5 0.25 |
1 1 |
32 32 |
27 27 |
30 31 |
| C. pararugosa (N = 1) | 17-442 | 0.125 | 0.25 | 8 | 0.25 | 0.25 | 2 | 34 | 26 | 29 |
| Nitroxoline quality control strains (N = 6) | ||||||||||
| ATCC 25922 Escherichia coli ATCC 10231 Candida albicans ATCC 90030 Candida glabrata ATCC 90877 Candida guilliermondii ATCC 6285 Candida krusei ATCC 22019 Candida parapsilosis |
2 0.25 0.5 0.25 0.5 1 |
22 35 34 35 32 29 |
23 32 28 29 29 27 |
23 32 31 31 31 26 |
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a
Bold numbers indicate MICs in the resistant range based on EUCAST breakpoints (version 10.0) or tentative CDC-breakpoints for C. auris (available at https://www.cdc.gov/fungal/candida-auris/c-auris-antifungal.html [last accessed 11/2021]). For Nitroxoline quality control and reproduction of nitroxoline susceptibility testing with yeasts elsewhere, test results of six different ATCC strains were included.