FIG 2.
Workflow for drug detection in pulmonary lesions of BALB/c IL-13tg mice. Infection of BALB/c IL-13tg mice with M. tuberculosis H37Rv, drug treatment after necrotizing granulomas have developed, and lung tissue preparation were performed in a BSL-3 laboratory. Inactivation of pulmonary M. tuberculosis by gamma irradiation was confirmed by the MGIT growth system before consecutive cryosections were processed for subsequent analyses, including histological characterization, drug distribution by MALDI-MS imaging, and drug quantification by LC-MS/MS (LC-MS/MS image created using Biorender).