TABLE 6.
Characteristics of HIV-1 virus preparations and assessment for replicative capacity in MT4-GFP cellsa
| HIV-1 variant | p24 (pg/mL)b | MT4-GFP normalized p24 inputc (pg/mL) | FCd | R0 by change in no. of GFP-positive cells | R0 by change in p24 level |
|---|---|---|---|---|---|
| WT | 7.55 × 105 | 3.93 × 103 | 1.0 | 18.66 | 5.44 |
| M184V | 7.63 × 105 | 7.95 × 103 | 2.0 | 21.43 | 6.20 |
| M184I | 6.51 × 105 | 8.68 × 103 | 2.2 | 22.23 | 7.39 |
| A114S/M184V | 6.02 × 105 | 2.51 × 104 | 6.4 | 5.69 | 3.42 |
| M41L/A114S/M184V | 4.45 × 105 | 7.42 × 103 | 1.9 | 7.54 | 3.70 |
a
FC, fold-change; HIV-1, human immunodeficiency virus type 1.
b
Concentrations of p24 were determined by titration of viral stocks across a wide range of dilutions and interpolation to a linear standard curve.
c
MT4-GFP normalized p24 input: the concentration of virus in pg/mL of p24 that was required to infect 8 × 104 MT4 GFP cells and detect approximately 300 GFP-positive cells 48 h after infection.
d
FC is calculated as (MT4-GFP normalized p24 input mutant)/(MT4-GFP normalized p24 input WT).