TABLE 1.
Characteristics of Nm512 and genetic transformation from Nei341 into Nm040
| Strain | Attribute of strain | Penicillin MIC (μg/mL) | penA allele (mutations) | Breakage point positiona |
Recombinant size (bp) | PubMLST ID | Reference | |
|---|---|---|---|---|---|---|---|---|
| Upstream | Downstream | |||||||
| Nm512 | Clinical isolate | 0.19 | penA832 (F504L, A510V, I515V, H541N, I566V) | +718 | +2094 | 1,377 | 71401 | 8 |
| Nei341 | Donor | 0.25 | penA832 (F504L, A510V, I515V, H541N, I566V) | 83066 | This study | |||
| Nm040 | Recipient | 0.032 | penA1 (none) | 58130 | 41 | |||
| Nm040Nei341T1-1 | Transformant | 0.19 | penA832 (F504L, A510V, I515V, H541N, I566V) | +757 | +2048 | 1,292 | 111265 | This study |
| Nm040Nei341T1-2 | Transformant | 0.19 | penA832 (F504L, A510V, I515V, H541N, I566V) | +328 | +8664 | 7,805 | 111266 | This study |
| Nm040Nei341T1-3 | Transformant | 0.19 | penA832 (F504L, A510V, I515V, H541N, I566V) | +328 | +5534 | 4,554 | 111267 | This study |
| Nm040Nei341T1-4 | Transformant | 0.19 | penA832 (F504L, A510V, I515V, H541N, I566V) | +328 | +5534 | 4,554 | 111268 | This study |
a
The first nucleotide of the initial codon of penA of Nm040 was recorded as +1.