| Antibodies |
|
|
|
| Mouse monoclonal anti-NimC antibody |
Dr. Istvan Ando |
RRID: AB_2568423 |
| Dorsal |
Developmental Studies Hybridoma Bank (DSHB) |
DSHB Cat# anti-dorsal 7A4; RRID:AB_528204 |
| Mmp1 |
DSHB |
DSHB Cat# 3A6B4; RRID:AB_579780 |
| Wingless |
DSHB |
DSHB Cat# 4d4; RRID:AB_528512 |
| Elav |
DSHB |
DSHB Cat# Rat-Elav-7E8A10 anti-elav; RRID:AB_528218 |
| Mouse monoclonal anti-β-Galactosidase |
DSHB |
DSHB Cat# 40–1a; RRID:AB_528100 |
| Cleaved Dcp-1 (Asp176) |
Cell Signaling Technology |
Cat# 9578; RRID:AB_2721060 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Dihydroethidium (DHE) |
Invitrogen |
Cat#D23107
|
| H2DCF-DA |
Molecular Probes |
Cat#C6827 |
| Vectorshield with DAPI |
Vector Laboratories |
Cat#H-1200; RRID: AB_2336790 |
| Hoechst 33342 |
Invitrogene |
Cat#H3570 |
|
| Experimental models: Organisms/strains |
|
|
|
|
Drosophila melanogaster: Toll-9rv17
|
this study |
N/A |
|
Drosophila melanogaster: UAS-Toll-9
|
this study |
N/A |
|
Drosophila melanogaster: UAS-Toll-9INTRA
|
this study |
N/A |
|
Drosophila melanogaster: UAS-Toll-9INTRA
|
this study |
N/A |
|
Drosophila melanogaster: UAS-Toll-9 RNAi
|
Bloomington Drosophila Stock Center (BDSC) |
BDSC 30535 FlyBase: FBst0030535 |
|
Drosophila melanogaster: UAS-Toll-9 RNAi
|
Vienna Drosophila Resource Center (VDRC) |
VDRC v36308 FlyBase: FBst0461624 |
|
Drosophila melanogaster: UAS-Toll-9 RNAi
|
VDRC |
VDRC v109635 FlyBase: FBst0481299 |
|
Drosophila melanogaster: UAS-Dif RNAi
|
VDRC |
VDRC v100537 FlyBase: FBst0472410 |
|
Drosophila melanogaster: UAS-dorsal RNAi
|
VDRC |
VDRC v105491 FlyBase: FBst0477317 |
|
Drosophila melanogaster: UAS-dorsal RNAi
|
BDSC |
BDSC 34938 FlyBase: FBst0034938 |
|
Drosophila melanogaster: UAS-MyD88 RNAi
|
VDRC |
VDRC v106198 FlyBase: FBst0478023 |
|
Drosophila melanogaster: UAS-pelle RNAi
|
VDRC |
VDRC v103774 FlyBase: FBst0475632 |
|
Drosophila melanogaster: UAS-Toll-1 RNAi
|
VDRC |
VDRC v100078 FlyBase: FBst0471952 |
|
Drosophila melanogaster: UAS-reaper RNAi
|
BDSC |
BDSC 51846 FlyBase: FBst0051846 |
|
Drosophila melanogaster: UAS-hid RNAi
|
VDRC |
VDRC v7912 FlyBase: FBst0470883 |
|
Drosophila melanogaster: UAS-dronc RNAi
|
VDRC |
FlyBase: FBst0472297; VDRC v100424 |
|
Drosophila melanogaster: UAS-bsk RNAi
|
VDRC |
VDRC v34138 FlyBase: FBst0460476 |
|
Drosophila melanogaster: UAS-Luciferase RNAi
|
BDSC |
BDSC 31603 FlyBase: FBst0031603 |
|
Drosophila melanogaster: 4kb rpr-lacZ
|
Hermann Steller |
FlyBase: FBal0105175 |
|
Drosophila melanogaster: hid[10–1kb]-lacZ
|
this study |
N/A |
|
Drosophila melanogaster: UAS-Toll-1
|
Tony Ip |
FlyBase : FBal0181209 |
|
Drosophila melanogaster: UAS-SPE RNAi
|
VDRC |
VDRC v104906 FlyBase: FBst0476734 |
|
Drosophila melanogaster: UAS-spzAct
|
Laura Johnson |
FlyBase: FBal0138129 |
|
Drosophila melanogaster: UAS-Duox RNAi (line #44)
|
Won-Jae Lee |
FlyBase: FBal0190061 |
|
Drosophila melanogaster: UAS-Nox RNAi (line #4)
|
Won-Jae Lee |
FlyBase: FBal0191562 |
|
Drosophila melanogaster: TRE-RFP
|
Dirk Bohmann |
FlyBase: FBst0059011 |
|
| Oligonucleotides |
|
|
|
| Toll-9, Forward: 5′CCATTACAAGCACTATAGG |
this study |
N/A |
| Toll-9, Reverse: 5′-GACCTCTTCGGCCTCTTC |
this study |
N/A |
| RP-49: Forward: 5′-CCAGTCGGATCGATATGCTAA |
this study |
N/A |
| RP-49: Reverse: 5′-ACGTTGTGCACCAGGAACTT |
this study |
N/A |
|
| Software and algorithms |
|
|
|
| Photoshop |
Adobe |
Version 2021 |
| GraphPad Prism Software |
GraphPad |
Version 9 |
| Zen imaging software |
Carl Zeiss AG |
Version 3.1 |
