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. 2022 Jun 13;11(6):bio059143. doi: 10.1242/bio.059143

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Haematopoiesis-specific deletion of PADI4 has no major impact on steady-state haematopoiesis. (A) Padi4 expression derived from five bulk sequencing studies and batch corrected, with each study contributing to an average of four populations. (B) Immunoblot analysis of mouse PADI4 in total BM extracts from Padi4^CTL and Padi4^CKO mice. Actin presented as a loading control. (C–H) Immunophenotypic analysis of bone marrow from 8–12-week-old mice; total number of (C) WBC, LSK, and LK cells (D) HSC, MPP, HPC-1 and HPC-2 cells. Padi4^CTL, n=9; Padi4^CKO, n=9. (E,F) Total number of lymphoid, myeloid and erythroid progenitor cells. (E) CLP, (F) CMP, GMP, MEP, Pre-GMP, Pre-MegE, MkP, Pre-CFU, CFU-E, Pro-Ery and differentiated cell populations (Granulocytes, Monocytes, Erythroid and B cells). Padi4^CTL, n=9; Padi4^CKO, n=9. (G) CFC assay with BM cells. Padi4^CTL, n=5; Padi4^CKO, n=6. (H) Immunophenotypic analysis of spleen from 8–12-week-old mice; total number of WBC, T cells and differentiated cell populations (granulocytes, monocytes, erythroid and B cells). Padi4^CTL, n=9; Padi4^CKO, n=9. All data are mean±s.e.m. *, P<0.05; **, P<0.01; ***, P<0.001, ****, P<0.0001 (Mann–Whitney U-test).