TABLE 1.
Screening of three environmental libraries for genes conferring lipolytic activity
| Test substrate | Library | Sample site | No. of E. coli clones tested | No. of lipolytic E. coli clones after the initial screen | No. of lipolytic E. coli clones with a stable phenotype (designation) |
|---|---|---|---|---|---|
| Triolein | I | Meadow | 430,000 | 0 | |
| II | Sugar beet field | 180,000 | 1 | 1 (pAH110) | |
| III | Nieme River valley | 120,000 | 0 | ||
| Tributyrin | I | Meadow | 73,000 | 1 | 1 (pAH111) |
| II | Sugar beet field | 115,000 | 0 | ||
| III | Nieme River valley | 98,000 | 2 | 2 (pAH112; pAH113) |