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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Hypertension. 2021 May 24;78(1):115–127. doi: 10.1161/HYPERTENSIONAHA.121.16770

Figure. 6.

Figure. 6.

The role and mechanism of sPRR in regulation of renin expression in cultured As4.1 and M-1 cells. The confluent cells were pretreated for 1 h with the β-catenin inhibitor ICG-001 at 10 μM and then treated for 24h with 10 nM sPRR-His. At the end of the experiment, cells and/or the media were harvested for qRT-PCR analysis of renin mRNA, enzyme activity-based assay for total renin content, and ELISA measurement of prorenin/renin content. (A) Renin mRNA expression in As4.1 cells. (B) Cellular total prorenin/renin content in As4.1 cells. (C) Cellular total renin content in As4.1 cells. (D) Renin mRNA expression in M-1 cells. (E) Cellular total prorenin/renin content in M-1 cells. (F) Cellular total renin content in M-1 cells. N = 5–8 per group. Data are mean ± SE.