Fig. 5.

RMC-76 inhibits temporally distinct stages of SARS-CoV-2 life cycle.

(A) Schematic of the time-of-addition experiments shown in panel B and C. Calu-3 cells were infected with rSARS-CoV-2/WT at an MOI of 1. At the indicated time points, 5 μM RMC-76 or DMSO were added to the infected cells. (B) Infectious virus titers measured by plaque assays in culture supernatants harvested at 10 hpi. (C) Intracellular SARS-CoV-2 nucleocapsid (N) copy numbers measured by RT-qPCR assays in cell lysates harvested at 10 hpi. Data are relative to DMSO and are representative of two independent experiments, each with three replicates, means ± SD are shown. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA followed by Dunnett's multiple comparisons test. Ns, non-significant.