Fig. 6.

NAKs are required for the entry of pseudovirus and WT SARS-CoV-2.

(A) Schematic of the experiments shown in panels B and C. (B) rVSV-SARS-CoV-2-S infection at 24 hpi of Calu-3 cells depleted of the indicated NAKs-by siRNAs (Fig. 1B and C) measured via luciferase assays. (C) rVSV-SARS-CoV-2-S entry at 2 hpi of Calu-3 cells (MOI 3) depleted of the indicated NAKs by siRNAs measured via RT-qPCR. (D) Schematic of the experiment shown in panel E. (E) rSARS-CoV-2/WT entry at 2 hpi of Calu-3 cells (MOI = 1) depleted of the indicated NAKs by siRNAs (Fig. 1B and C) measured via RT-qPCR. Data in all panels are representative of two or more independent experiments. Individual experiments had three biological replicates, means ± SD are shown. Data are relative to siNT (B, C, E). ***P < 0.001; ****P < 0.0001 by 1-way ANOVA followed by Dunnett's multiple comparisons test.