TABLE 2.
Characteristics of methylthio-s-triazine substrates and of metabolites accumulated after overnight incubation of suspensions of Nocardioides sp. strain C190a
| Substrate | Characteristics of substrate
|
Characteristics of product
|
||
|---|---|---|---|---|
| HPLC retention time (min) | m/z (% intensity) | HPLC retention time (min) | m/z (% intensity) | |
| Atrazine | 7.2 | 215 (M+, 75), 200 (100), 173 (20), 132, 18, 71 (24), 58 (58) | 4.6 | 197 (M+, 100), 182 (90), 155 (45), 127 (36), 112 (80), 69 (52), 58 (92) |
| Terbuthylazine | 7.9 | 229 (40), 214 (M+, 100), 173 (50), 132 (30), 71 (42) | 5.4 | 211 (80), 196 (M+, 100), 169 (12), 155 (80), 126 (72), 112 (30), 69 (30), 58 (42) |
| Simetryn | 8.1 | 213 (M+, 100), 198 (24), 170 (50), 155 (42), 96 (35), 71 (53), 68 (84) | 4.0 | 183 (M+, 10), 149 (22), 111 (15), 85 (45), 70 (55), 55 (100) |
| Ametryn | 8.7 | 227 (M+, 100), 212 (64), 185 (35), 170 (40), 99 (25), 68 (60), 58 (64) | 4.6 | 197 (M+, 100), 182 (90), 155 (35), 127 (25), 112 (62), 69 (70), 57 (96) |
| Prometryn | 9.3 | 241 (M+, 100), 226 (60), 199 (30), 184 (72), 106 (25), 58 (64) | 4.8 | 211 (M+, 100), 196 (94), 169 (42), 154 (58), 112 (65), 69 (45), 58 (88) |
| Terbutryn | 15.0 | 241 (M+, 70), 226 (90), 185 (100), 170 (55), 106 (32), 68 (60) | 5.8 | 211 (M+, 62), 196 (100), 155 (72), 126 (64), 69 (28) |
a
The initial substrate concentration was 10 mg/liter.