TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| P. fluorescens strains | ||
| CHA0 | Ant+ HPT+ Flu+ DS+ Cmr, wild type | 15 |
| CHA89 | Ant− HPT− Flu++ DS− Kmr, gene replacement gacA mutant of CHA0 | 21 |
| CHA96rif | Ant− HPT− Flu++ DS− Kmr, Rifr derivative of CHA89 | 26 |
| CHA510 | Ant− HPT− Flu++ DS− Kmr, apdA::Tn5 mutant of CHA0 | Bull and Haasb |
| CHAS9 | Ant− HPT− Flu++ DS−, spontaneous gacA mutant of CHA0 | Bull and Haasb |
| CHAS45 | Ant− HPT− Flu++ DS−, spontaneous gacA mutant of CHA0 | This study |
| CHAS17 | Ant− HPT− Flu++ DS−, spontaneous apdA mutant of CHA0 | Bull and Haasb |
| CHASP1 | Ant− HPT− Flu++ DS−, spontaneous apdA mutant of CHA0 | This study |
| PGNL1 | Ant+ HPT+ Flu+ DS+, wild type | 16 |
| PGNL1S2 | Ant− HPT− Flu++, spontaneous gacA mutant of PGNL1 | This study |
| PGNR1 | Ant+ HPT+ Flu+ DS+, wild type | 16 |
| PGNR1S1 | Ant− HPT− Flu++, spontaneous gacA mutant of PGNR1 | This study |
| PGNR4 | Ant+ HPT+ Flu+ DS+, wild type | 16 |
| PGNR4S2 | Ant− HPT− Flu++, spontaneous gacA mutant of PGNR4 | This study |
| PINR2 | Ant+ HPT+ Flu+ DS+, wild type | 16 |
| PINR2S3 | Ant− HPT− Flu++, spontaneous gacA mutant of PINR2 | This study |
| PINR3 | Ant+ HPT+ Flu+ DS+, wild type | 16 |
| PINR3S1 | Ant− HPT− Flu++, spontaneous gacA mutant of PINR2 | This study |
| E. coli strains | ||
| DH5α | F−endA1 hsdR17 (rK−mK+) supE44 thi-1 recA1 gyrA96 relA1 φ80dlacZΔM15λ− | 31 |
| ED8767 | metB hsdS supE supF recA56 | 25 |
| HB101 | F−hsdS20 supE44 recA13 ara14 proA2 lacY1galK2 rpsL20 leu-6 thi-1 xyl-5 mtl-1 (str-20)Δ(mcrC-mrr) | 2 |
| Plasmids | ||
| pME3066 | IncP-1 replicon, Mob+ Tcr, contains a functional gacA (Y49) gene from CHA0 | 21 |
| pJEL5771 | Tcr, contains a functional apdA gene from P. fluorescens Pf-5 | 6 |
| pEMH97 | Tcr, contains a functional lemA gene from P. syringae pv. syringae B728a | 13 |
| pME497 | IncP-1 replicon, RepA(ts) Tra+ Kmr | 38 |
a
Ant, antibiotics (PHL and PLT); HPT, hydrogen cyanide, protease, TSO; DS, disease suppression; Flu, fluorescent siderophores (pyoverdine and pyochelin) (+, wild-type production level; ++, overproduction; −, reduced or no production); Apr, Cmr, Kmr, Tcr, and Rifr, resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, and rifampin, respectively.
b
Bull and Haas, unpublished data.