TABLE 5.
Effects of liquid media on bacterial growtha
| Medium | Conductivity (mS) | Log CFU/mlb
|
Fisher's LSD0.05 | ||||
|---|---|---|---|---|---|---|---|
| CHA0 | CHAS17 | CHASP1 | CHAS9 | CHAS45 | |||
| NBY broth | 2.4 | 9.67 | 9.48 | 9.57 | 9.39 | 9.55 | 0.15 |
| NBY broth containing CuSO4 | 2.4 | 9.66 | 9.52 | 9.54 | 9.28 | 9.00 | 0.16 |
| NBY broth containing ZnSO4 | 2.3 | 9.61 | 9.58 | 9.69 | 9.19 | 9.60 | 0.20 |
| NBY broth containing CoCl2 | 2.4 | 9.37 | 9.46 | 9.36 | 9.38 | 9.45 | NS |
| NBY broth containing Mo7(NH4)6O24 | 2.9 | 9.59 | 9.55 | 9.67 | 9.44 | 9.66 | 0.11 |
| NBY broth containing MnCl2 | 2.3 | 9.68 | 9.49 | 9.63 | 9.40 | 9.57 | 0.13 |
| NBY broth containing LiCl | 2.4 | 9.61 | 9.53 | 9.61 | 9.39 | 9.51 | 0.16 |
| NBY broth containing FeSO4 | 2.3 | 9.54 | 9.50 | 9.59 | 9.38 | 9.57 | 0.12 |
| NBY broth containing BH3O3 | 2.2 | 9.65 | 9.58 | 9.63 | 9.44 | 9.58 | 0.13 |
| NBY broth containing MgSO4 | 2.4 | 9.67 | 9.53 | 9.68 | 9.51 | 9.48 | 0.14 |
| NBY broth containing NaCl | 2.3 | 9.67 | 9.48 | 9.59 | 9.35 | 9.57 | 0.18 |
| NBY broth containing CaCl2 | 2.4 | 9.67 | 9.56 | 9.65 | 9.51 | 9.53 | 0.11 |
| Dilute (0.1×) NBY broth | 0.2 | 8.86 | 8.86 | 8.94 | 8.82 | 8.89 | 0.07 |
| Dilute (0.1×) NBY broth containing 30 mM NaCl | 4.0 | 8.93 | 8.89 | 8.98 | 8.85 | 9.01 | 0.08 |
Wild-type strain CHA0, gacS mutants CHAS17 and CHASP1, and gacA mutants CHAS9 and CHAS45 were inoculated individually into 20 ml of NBY broth, NBY broth amended with a mineral, or dilute NBY broth. Sodium chloride was added to dilute NBY broth to increase the medium conductivity. Total viable cell counts (log CFU) were determined after 48 h.
The values are means based on six replicates. The medium-strain interaction was significant (P ≤ 0.0001), and data were analyzed on the basis of the response to medium (P ≤ 0.0001 within a column) and strain (P ≤ 0.0429 within a row, except where NS indicates that the differences were not significant as determined by analysis of variance). The means were compared by using Fisher's protected LSD test. The LSD0.05 values for strains CHA0, CHAS17, CHASP1, CHAS9, and CHAS45 were 0.10, 0.14, 0.13, 0.16, and 0.14, respectively.