REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial strains
Escherichia coli strain DH5α	Vazyme	Cat# C502-02
Escherichia coli strain BL21 (DE3)	Vazyme	Cat# C504-02
Escherichia coli strain DH10Bac	Gibco	Cat# 10361012
Chemicals, antibodies and recombinant proteins
Polyethylenimine (PEI)	Polysciences	Cat# 24885-2
Anti-His/APC	Miltenyi Biotec	Cat# 130-119-820; RRID:SCR_008984
FuGENE 6 Transfection Reagent	Promega	Cat# E2691
BaseMuncher endonuclease	Abcam	Cat# ab270049
SARS-CoV-2 prototype RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_402119
SARS-CoV-2 Alpha RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_683466
SARS-CoV-2 Beta RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_678615
SARS-CoV-2 Gamma RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_833172
SARS-CoV-2 Delta RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_2020954
SARS-CoV-2 Omicron BA.1 RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_6640916
SARS-CoV-2 Omicron BA.1.1 RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_ 496747
SARS-CoV-2 Omicron BA.2 RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_9845731
SARS-CoV-2 Omicron BA.3 RBD protein with His-tag, spike residues 319-541	This paper	accession number: EPI_ISL_7605713
Human ACE2 protein, residues 19-615	This paper	accession number: NP_001358344
Critical commercial assays
HisTrap HP 5 mL column	GE Healthcare	Cat# 17524802
Superdex 200 Increase 10/300 GL	GE Healthcare	Cat# 28990944
HiLoad 16/600 Superdex 200 pg	GE Healthcare	Cat# 28989335
Series S Sensor Chip SA	GE Healthcare	Cat# 29104992
Membrane concentrator	Millipore	Cat# UFC901096
Membrane concentrator	Millipore	Cat# SLHP033RB
Deposited data
Omicron BA.1.1 RBD/hACE2 complex (X-RAD)	This paper	PDB: 7XAZ
Omicron BA.2 RBD/hACE2 complex (X-RAD)	This paper	PDB: 7XB0
Omicron BA.3 RBD/hACE2 complex (X-RAD)	This paper	PDB: 7XB1
Experimental models: Cell lines
HEK293F cells	Gibco	Cat# 11625-019
HEK293T cells	ATCC	ATCC CRL-3216
BHK-21 cells	ATCC	CCL-10
Sf9 cells	Gibco	Cat# 11496015
Recombinant DNA
pEGFP-N1	MiaoLingPlasmid	Cat# P0133
pCAGGS	MiaoLingPlasmid	Cat# P0165
pFastBac1	Invitrogen	Cat# 10359-016
Software and algorithms
PyMOL software	Molecular Graphics System, Version 1.8 Schrö dinger	https://pymol.org/2/
Graphpad Prism 8	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
BIAcore 8K Evaluation software	GE Healthcare	N/A
FlowJo V10	FLOWJO	https://www.flowjo.com/solutions/flowjo/downloads
HKL2000	HKL Research	https://www.hkl-xray.com/hkl-2000
COOT	(Emsley and Cowtan, 2004)	http://www.mrc-lmb.cam.ac.uk/personal/peemsley/coot/
Phenix	(Adams et al., 2010)	http://www.phenix-online.org/
MolProbity	Duke Biochemistry	http://molprobity.biochem.duke.edu/index.php
GROMACS	(Abraham et al., 2015)	http://www.gromacs.org/