Table 5.

Comparison of RT-ddPCR and WGS results for clinical specimens at each of four amino acids. The number of specimens with a given genotype based on RT-ddPCR (rows) and WGS (columns) is listed for each assay. For each mutation, the Positive Predictive Value (PPV) and Negative Predictive Value (NPV) are calculated based on this comparison.

		WGS			Total			WGS
		417K	417N	417T				484E	484K	484Q	Total
ddPCR	417K	359	0	2	361	ddPCR	484E	322	0	0	322
	417N	0	23	0	23		484K	1	79	0	80
	417T	0	0	35	35		484Q	0	0	17	17
	Total	359	23	37	419		Total	323	79	17	419
		PPV:	100	100				PPV:	100	100
		NPV:	100	99.5				NPV:	99.7	100

		WGS					WGS
ddPCR		452L	452R	Total	ddPCR		501N	501Y	Total
	452L	319	0	319		501N	149	0	149
	452R	0	100	100		501Y	2	268	270
	Total	319	100	419		Total	151	268	419
		PPV:	100				PPV:	99.3
		NPV:	100				NPV:	98.7