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Construction of plasmids encoding tRNAs. The ileX, argU, and leuW sequences encode tRNAs that recognize the ATA, AGA, and CTA codons, respectively. The Apr, Tetr, and Cmr sequences encode genes for antibiotic resistance. The T7 and Sp6 sequences encode promoters from bacteriophages T7 and sp6, respectively. The shaded areas represent sequences of subB-E tRNA operons other than leuW. DNA pol Taq, Taq DNA polymerase; DNA polT4, T4 DNA polymerase.
