TABLE 1.
Oligonucleotides used
| Primer | Sequence | Amplified sequencea |
|---|---|---|
| 5′-Ile-tRNA | 5′-AAGCTTTGGATTGCGACACGGAGTTACTTT | ileX |
| 3′-Ile-tRNA | 5′-GCTTTTGATCTCTCGAGAAAAGAAAAAGGCTGACGATTTCTCGTCAGC | ileX |
| 5′-Arg-tRNA | 5′-CTTTTTCTTTTCTCGAGAGATCAAAAGCCATTGACTCAGCA | argU |
| 3′-Arg-tRNA | 5′-GTCGACTCAGGCGTCCCATTATCAGTG | argU |
| Leu-5′ | 5′-AACACAAAGTCGACAATAATTGACGAATATAGCGCC | subB-E |
| Leu-3′ | 5′-GTCAACATCGCGGCCGACATTGAATGAACGC | subB-E |
| BoNT/A-N | 5′-ATAAGAGGATCCGCGGATGCAATTTGTTAATAAACAATTTAATT | BoNT/A-L |
| BoNT/A-LC | 5′-TATCTTCTGAGAATTCTTATGTCGACATCCAATTGTTAACTTTGATACATAAATC | BoNT/A-L |
| BoNT/B-N | 5′-GGATCCGCGGATGCCAGTTACAATAAATAATTTTAATT | BoNT/B-L |
| BoNT/B-LC | 5′-GAATTCTTATGTCGACATACATATTCCTGGAGCTTTAAC | BoNT/B-L |
| BoNT/B-HN | 5′-CCATGGGACATCATCACCATCACCACGGGGATCCACAAGCTTATGAAGAAATTAGCAA | BoNT/B-H |
| BoNT/B-HC | 5′-GAATTCGGATCCTATTATTCAGTCCACCCTTCAT | BoNT/B-H |
| BoNT/C-N | 5′-GGATCCGCGGATGCCAATAACAATTAACAACTTTAATTATT | BoNT/C-L |
| BoNT/C-LC | 5′-GAATTCTTATGTCGACCTACAATCTAATGTTTTATTATA | BoNT/C-L |
| BoNT/C-HN | 5′-GGATCCTGTACAAAATAGGAAAATATATCTTTC | BoNT/C-H |
| BoNT/C-HC | 5′-AGATCTTATTCACTTACAGGTACAAAACC | BoNT/C-H |
| BoNT/E-N | 5′-GGATCCGCGGATGCCAAAAATTAATAGTTTTAATTATA | BoNT/E-L |
| BoNT/E-LC | 5′-GAATTCTTATGTCGACATACATATTGATTTCCTTATGCC | BoNT/E-L |
| BoNT/E-HN | 5′-GGATCCAAATTTAAATCCTAGAATTATTACACCAA | BoNT/E-H |
| BoNT/E-HC | 5′-AGATCTTATTTTTCTTGCCATCCATGTTCTT | BoNT/E-H |
| TetLN | 5′-GGAGATGATACATATGCCAATAACCATAAATAATT | TeNT-L |
| tet-LC | 5′-AAGTTAAATCAAGCTTTTATGTCGACATACATAATTCTCCTCCTAAATCTGT | TeNT-L |
| TetCN | 5′-TGCTTTTAGACATATGGATGGATCAGGCCTAGTTT | TeNT-H |
| TetHC | 5′-TGAACATATCAAGCTTTTTAATCATTTGTCCATCC | TeNT-H |
| iota/IaN | 5′-ATTATATTACGGATCCAGCTTTTATTGAAAGACCAGAAG | Iota Ia |
| iota/IaC | 5′-ATTTATATTACTCGAGTTAATTTATCAATGTTGCATCCAAAAT | Iota Ia |
| N-C3 | 5′-GGATCCAGGAGGGGTTTTATGAAAGGGATAAGAAAGTCAATTTTATGTTTAG | C3 |
| IC3-C | 5′-AGATCTGAATTCTTAAATATCATTGCTGTAATCATAAT | C3 |
| Ile1 | 5′-GGCCCCTTAGCTCAGTGGTT | Ile-tRNA |
| Ile2 | 5′-CCCCTGCTGGACTTGAACCA | Ile-tRNA |
| Arg1 | 5′-GCGCCCTTAGCTCAGTTGGA | Arg-tRNA |
| Arg2 | 5′-TGGCGCGCCCTGCAGGATTC | Arg-tRNA |
| Leu1 | 5′-GCGGGAGTGGCGAAATTGGT | Leu-tRNA |
| Leu2 | 5′-TGGTGCGGGAGGCGAGACTT | Leu-tRNA |
| Ile10 | 5′-TATGATAATAATAATAATAATAATAATAATAATATCGAGCT | NA |
| Ile10-comp | 5′-CGATATTATTATTATTATTATTATTATTATTATCA | NA |
| Arg10 | 5′-TAGTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATCGAGCT | NA |
| Arg10-comp | 5′-CGATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAC | NA |
| Leu10 | 5′-TATGCTACTACTACTACTACTACTACTACTACTATCGAGCT | NA |
| Leu10-comp | 5′-CGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGCA | NA |
a
TeNT-L, light chain of tetanus toxin; TeNT-H, heavy chain of tetanus toxin; NA, not applicable.