REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Spike of SARS-CoV-2	Sino biological	MA14AP0204
Alexa Fluor 488 Goat anti-rabbit	Thermo Fisher	Cat # A-11034
Anti-FLAG M2 agarose affinity gel (For IP)	Sigma-Aldrich	A2220
Anti-acety-lysine (For IP)	ImmuneChem	ICP0380
Anti-FLAG M2 HRP conjugated (For WB)	Sigma-Aldrich	A8592
Anti-Brd4 (For WB)	Abcam	Ab46199
Bacterial and virus strains
Escherichia coli BL21-CodonPlus (De3) RIL	Agilent Technologies	230245
DH5α Competent Cells	Thermo Fisher Sci	Cat. # 18265017
SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05647/2021	BEI Resources, NIAID, NIH	NR-55672
Chemicals, peptides, and recombinant proteins
Dithiothreitol	Gold Biotechnology	27565-41-9
β-mercaptoethanol	Sigma-Aldrich	Cat. # M6250
Imidazole	Alfa Aesar	Cat. # A10221
15NH4Cl	Sigma-Aldrich	Cat. # 299251
JQ1	Cayman Chemical	11187
OTX015	Cayman Chemical	15947
IPTG	Goldbio	Cat. # I2481
Ni-NTA resin	Thermo Scientific	Cat. # 88223
Glutathione Sepharose 4B beads	GE Healthcare	Cat. # 17-0756-01
Streptavidin magnetic beads	Thermo Fisher Sci	Cat. # 88816
E peptides	Synpeptide	N/A
PreScission protease	Home expressed	N/A
Thrombin protease	MP Biomedicals	154163
Enterokinase protease	NEB	P8070S
Hoechst 33258	Invitrogen	Cat # H3570
Cell Mask	Invitrogen	Cat # C10046
Recombinant p300 (catalytic domain)	Active Motif	81093
Lipofectamine 3000	Invitrogen	L3000001
Critical commercial assays
Platinum™ II Hot-Start PCR Master Mix (2X)	Thermo Fisher Sci	14000013
QIAprep Spin Miniprep Kit	QIAGEN	Cat # 27106
Deposited data
Crystal structure of BRD4 Bromodomain 1 in complex with SARS-CoV-2 E peptide diacetylated at K53 and K63	This study	PDB: 7TV0
Crystal structure of BRD4 Bromodomain 1 in complex with SARS-CoV-2 E peptide acetylated at K63	This study	PDB: 7TUQ
Experimental models: Cell lines
Calu-3 cells	ATCC, Manassas, VA, USA	HTB-55
K562	ATCC	CCL-243
293T	ATCC	CRL-3216
Oligonucleotides
BamH1 E: atataggatccTACAGCTTCGTATCAGAAGA	This study	N/A
Xho1 E: atatactcgagTCATTCGAGAACGAGGAGATC	This study	N/A
gBlockE: TACAGCTTCGTATCAGAAGAAA CCGGGACACTGATCGTAAATTCTGTGCTC TTGTTTCTGGCATTCGTCGTATTTC TCCTCGTCACACTGGCAATTCTGA CTGCATTGAGGCTTTGCGCCTACT GTTGTAACATTGTCAATGTATCTCTC GTGAAACCCTCATTCTACGTTTACA GCAGGGTGAAGAATCTCAATTCTA GCAGGGTGCCGGATCTCCTCGTTCTCGAA	This study	N/A
E_Sarbeco_F1: ACAGGTACGTTAATAGTTAATAGCGT	This study	N/A
E_Sarbeco_R2: ATATTGCAGCAGTACGCACACA	This study	N/A
E_Sarbeco_P1:FAM -ACACTAGCCATCCT TACTGCGCTTCG - BHQ1	This study	N/A
Recombinant DNA
pGEX-6P-1	Cytiva	28-9546-48
pGEX-4T-1	Cytiva	28-9545-49
pcDNA3.1	Invitrogen	V79020
Software and algorithms
XDS	Kabsch (2010)	https://xds.mr.mpg.de/
Phenix	Adams et al., 2010	http://www.phenix-online.org/
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Prism 9.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
CCP4	M. D. Winn et al., 2011	https://www.ccp4.ac.uk/
NMRPipe	Delaglio et al., 1995	https://www.ibbr.umd.edu/nmrpipe/
MO.Affinity Analysis	NanoTemper Technologies	https://nanotempertech.com/monolith-mo-control-software/
MO.Control software	NanoTemper Technologies	https://nanotempertech.com/monolith-mo-control-software/
Other
qPCR Control RNA from Heat-Inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020	BEI Resources, NIAID, NIH	NR-52347
HiPrep™ 16/60 Sephacryl® S-100 HR column	GE Healthcare	Cat. # 17-1165-01
HiTrap SP HP column	Cytiva	17115201
HiLoad Superdex 75 pg column	Cytiva	28989333