Table 1.
Regression parameters and validation sheet for determination of pure VAL, AML and HCT by the proposed methods.
| Drug | VAL | AML | HCT |
|---|---|---|---|
| Method |
D0 (λmax250.0 nm) |
D0 (λmax237.5 nm) |
D0 (λmax270.5 nm) |
|
Linearity range (µg/mL) |
4.0–44.0 | 4.0–40.0 | 2.0–24.0 |
| Regression equation Parameters | |||
| Slope | 0.0318 | 0.0366 | 0.068 |
| Intercept | 0.0112 | 0.002 | 0.0062 |
| Correlation Coefficient (r) | 1.0000 | 1.0000 | 0.9999 |
|
Accuracya (Mean ± SD) |
100.19 ± 0.59 | 99.67 ± 0.59 | 100.03 ± 0.89 |
|
Precision (±%RSD) | |||
| Repeatabilityb | 0.482 | 0.585 | 0.660 |
| Intermediate precision c | 0.654 | 0.850 | 0.896 |
a
Accuracy was checked using concentrations (6.0 µg/mL, 24.0 µg/mL and 40.0 µg/mL), (6.0 µg/mL, 25.0 µg/mL and 38.0 µg/mL) and (4.0 µg/mL, 16.0 µg/mL and 23.0 µg/mL) for, VAL, AML and HCT respectively.
b
and c are repeatability and intermediate precision respectively (n = 9) relative standard deviation of three different concentrations in triplicate (8.0 µg/mL, 28.0 µg/mL and 36.0 µg/mL) for both VAL and AML, while (6.0 µg/mL, 14.0 µg/mL and 22.0 µg/mL) for HCT.