Table 2.
Limits of detection and quantitation calculated for the diagnostic NCAP peptides on the existing LC-MS system spiked into various matrices are represented below.
| Protein | Matrix | Peptide | LOD (fmol on column) | LOQ (fmol on column) |
|---|---|---|---|---|
| NCAP | 0.1% Formic acid | AYNVTQAFGR | 0.17 | 0.85 |
| ADETQALPQR | 0.37 | 1.1 | ||
| KADETQALPQR | 0.26 | 1.3 | ||
| Artificial sputum | AYNVTQAFGR | 1.6 | 8.0 | |
| ADETQALPQR | 0.66 | 3.3 | ||
| KADETQALPQR | 6.3 | 32.1 | ||
| Saliva | AYNVTQAFGR | 2.6 | 12.8 | |
| ADETQALPQR | 4.7 | 23.6 | ||
| KADETQALPQR | 7.3 | 36.4 | ||
| Swab | AYNVTQAFGR | 1.5 | 7.2 | |
| ADETQALPQR | 3.0 | 14.8 | ||
| KADETQALPQR | 4.4 | 21.9 |