Appendix 1—table 1. qPCR primers used for genotyping and qRT-PCR analysis.

Target	Forward primer	Reverse primer	citation
Cre (genotyping)	TCCAATTTACTGAC CGTACACCAA	CCTGATCCTGGC AATTTCGGCTA
LSL-MAF1 (genotyping)	TTCACTTCATAC CCATACGACG	CCATTTTCCTTA TTTGCCCCTA
WT Maf1	AGGCTTGCAGG GCAGCAATG	CACTGGCTGACA GGGAGATG	Bonhoure et al., 2015
Maf1 KO (genotyping)	AGGCTTGCAGG GCAGCAATG	TGGCCCTTAGAG CTGGAGTG	Bonhoure et al., 2015
Pre-tRNALeu	GTCAGGATGGCC GAGTGGTCTAAG	CCACGCCTCCATACGGA GAACCAGAAGACCC	Chen et al., 2018
Pre-tRNAiMet	CTGGGCCCAT AACCCAGAG	TGGTAGCAGA GGATGGTTTC	Chen et al., 2018
Pre-tRNAIle	GTTAGCGCGC GGTACTTATA	GGATCGAACT CACAACCTCG	Graczyk et al., 2015
Pre-tRNAPro	GGCTCGTTGGTCTAGGG	TTTGAACCCGGGACCTC	Graczyk et al., 2018
Maf1	GACTATGACTTC AGCACAGCC	CTGGGTTATAGC TGTAGATGTCAC	Chen et al., 2018
Brf1	GGAAAGGAATCAAG AGCACAGACCC	GTCCTCGGGTAA GATGCTTGCTT	Chen et al., 2018
Runx2	AGGGACTATGG CGTCAAACA	GGCTCACGT CGCTCATCTT	Fujioka-Kobayashi et al., 2016
Col1a1	CCCAATGGTG AGACGTGGAA	TTGGGTCCCT CGACTCCTAC
Sp7	ATGGCGTCCT CTCTGCTTG	GTCCATTGGT GCTTGAGAAGG	Fitter et al., 2017
Bglap	TCTGACAAAG CCTTCATGTCC	AAATAGTGATA CCGTAGATGCG	Pustylnik et al., 2013
Alp	CGGATCCTGA CCAAAAACC	TCATGATGT CCGTGGTCAAT
Ibsp	GAAAATGGAG ACGGCGATAG	CATTGTTTTC CTCTTCGTTTGA
EF1a	CTGAACCATC CAGGCCAAAT	GGCTGTGT GACAATCCAG	Van Itallie et al., 2006
β-actin	CGACAACGGC TCCGGCATG	CTGGGGTGTTGAA GGTCTCAAACATG
Rankl	CAGCCATTTGC ACACCTCAC	GTCTGTAGGT ACGCTTCCCG
Opg	AGGAACTGCA GTCCGTGAAG	ATTCCACACT TTTGCGTGGC
Ppia1	CGAGCTGTTTGCAG ACAAAGTTCC	CCCTGGCACA TGAATCCTGG	Chen et al., 2018
Pparg	ATCATCTACACG ATGCTGGCCT	TGAGGAACTCC CTGGTCATGAATC	Chen et al., 2018
Pparg2	TCGCTGATGCA CTGCCTATGA	GGAGAGGTC CACAGAGCTGAT
Cebpa	GAACAGCAACGA GTACCGGGTA	CCATGGCCTT GACCAAGGAG	Chen et al., 2018
Fabp4	TGGGAACCTG GAAGCTTGTCT	TCGAATTCCAC GCCCAGTTTGA	Chen et al., 2018