Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
Rosa26-Lox-stop-lox-MAF1-HA; LSL-MAF1 |
This paper | An engineered construct of Rosa26-Lox-stop-lox-MAF1-HA was injected into C57Bl6/J mice embryonic stem cells. chimeric mice were created by by blastocyst injection of homologous recombinant clones. | |
| Strain, strain background (M. musculus) | Maf1-/- | Bonhoure et al., 2015 | Mouse line maintained in Dr. I Willis lab. | |
| Strain, strain background (M. musculus) | Prrx1 Cre | Jackson laboratory | Strain #:005584 | |
| Cell line (M. musculus) | ST2 | RIKEN cell bank | #RCB0224 | |
| Transfected construct (M. musculus) | Scramble shRNA | Addgene, Sheila Steward | #17,920 | Lentiviral construct to express shRNA |
| Transfected construct (M. musculus) | MAF1 shRNA#1 | Millipore sigma | TRCN0000125776 | Lentiviral construct to express shRNA |
| Transfected construct (M. musculus) | MAF1 shRNA#2 | Millipore sigma | TRCN0000125778 | Lentiviral construct to express shRNA |
| Transfected construct (M. musculus) | Brf1 shRNA#1 | Millipore sigma | TRCN0000119897 | Lentiviral construct to express shRNA |
| Transfected construct (M. musculus) | Brf1 shRNA#2 | Millipore sigma | TRCN0000119901 | Lentiviral construct to express shRNA |
| Transfected construct (M. musculus) | pInducer20 | Addgene Stephen Elledge | #44,012 | Lentiviral construct to express shRNA |
| Transfected construct (Human) | pInducer20-MAF1-HA | This paper | pInd20-MAF1-HA was cloned by taking MAF1-HA from pFTREW-MAF1-HA into a pInducer20 construct by gateway cloning using LR clonase. Cell line M. musculus construct: human | |
| Chemical compound, drug | Calcein | Millipore Sigma | C0875 | 10 mg/kg |
| Chemical compound, drug | Xylenol orange | Millipore Sigma | X0127 | 90 mg/kg |
| Chemical compound, drug | LR clonase | Thermo Fisher | #11791020 | |
| Chemical compound, drug | Doxycycline hyclate | Millipore Sigma | #D9891 | Used at 1 µM |
| Chemical compound, drug | ML-60218 | Millipore Sigma | #557,403 | RNA pol III inhibitor |
| Chemical compound, drug | Ascorbic acid | Sigma | #A4544 | Used at 50 µg/mL |
| Chemical compound, drug | Β-glycerolphosphate | Millipore Sigma | #35,675 | Used at 10 mM |
| Chemical compound, drug | Cetylpyridinium chloride | Sigma | #C0732 | Used at 10% for alizarin red extraction |
| Chemical compound, drug | rosiglitazone | Sigma | R2408 | Used at 1 µM |
| Chemical compound, drug | 3-isobutyl-1-methyl xanthine | Sigma | I5879 | Used at 0.5 mM |
| Chemical compound, drug | dexamethasone | Sigma | D4902 | Used at 2 µM |
| Chemical compound, drug | Insulin | Sigma | I05016 | Used at 10 µg/mL |
| Chemical compound, drug | RNA stat-60 | Tel-test Inc | #NC9256697 | |
| Chemical compound, drug | Alizarin Red | Sigma | #A5533 | Used at 1% at ph 4.2 |
| Chemical compound, drug | Oil red O | Sigma | #01391 | Used at 0.3% |
| Chemical compound, drug | collagenase IV | Gibco | #17104019 | Used at 2.5% |
| Commercial assay or kit | TRAP staining kit | Sigma | #387A-1KT | |
| Commercial assay or kit | Von Kossa staining | Statlab | #KTVKO | |
| Commercial assay or kit | Alkaline phosphatase staining | Vector laboratories | #SK5300 | |
| Commercial assay or kit | Quick-RNA miniprep kit | Zymo | #R1055 | Used for RNA isolation from cell culture |
| Commercial assay or kit | Direct-zol RNA miniprep kit | Zymo | #R2052 | Used for RNA isolation from femurs |
| Commercial assay or kit | Superscript IV First Strand Synthesis Kit | Invitrogen | #18091050 | cDNA synthesis |
| Commercial assay or kit | SYBR fast qPCR mastermix | KAPA Biosystems | #KK4602 | |
| Peptide, recombinant protein | M-CSF | Peprotech | #300–25 | Used at 30 ng/mL |
| Peptide, recombinant protein | RANK-L | Peprotech | #310–01 C | Used at 100 ng/mL |
| Peptide, recombinant protein | FGF2 | Biovision | #4,038 | Used at 10 ng/mL |
| Commercial assay or kit | DC protein assay | Biorad | #5000112 | |
| Antibody | Anti-MAF1 (H2) (mouse monoclonal) |
Santa Cruz | #SC-515614 | (Wb 1:500) |
| Antibody | Anti-TFIIIB90 (mouse monoclonal) |
Santa Cruz | #SC-390821 | Antibody to Brf1. (Wb 1:1000) |
| Antibody | Anti-VINCULIN (mouse monoclonal) |
Santa Cruz | # sc-73614 AF488 | (Wb 1:5000) |
| Antibody | Anti-RUNX2 (rabbit monoclonal) |
Cell Signaling | #12,556 | (Wb 1:1000) |
| Antibody | Anti-PPARγ (rabbit monoclonal) |
Cell Signaling | #2,435 | (Wb 1:1000) |
| Antibody | Anti-FABP4 (rabbit monoclonal) |
Cell Signaling | #3,544 | (Wb 1:1000) |
| Antibody | Anti-HA (Rat monoclonal) |
Roche | #11867423001 | (Wb 1:1000) |
| Software, algorithm | R- studio | https://rstudio.com | Version 4.1.1 | |
| Software, algorithm | DeSeq2 | 10.18129/B9.bioc.DESeq2 | ||
| Software, algorithm | clusterProfiler | doi.org/10.1016 /j.xinn.2021.100141 | ||
| Software, algorithm | InteractiVenn | 10.1186 /s12859-015-0611-3 | ||
| Software, algorithm | Graphpad prism | https://www.graphpad.com/ | Version 9.3.1 |