TABLE 3.
Cell lysis and PepX activity of suspensions of exponentially growing L. lactis MG1363 or MG1363acmAΔ1 after 3 h of incubation at 30°C in the presence of bacteriocin
| Strain | Bacteriocin added (300 AU/ml) | Lysis (%)a | PepX (U/ml)b |
|---|---|---|---|
| L. lactis MG1363 | Nonec | 1 | 0.001 |
| Bacteriocin of L. lactis IFPL105 | 17.5 | 0.007 | |
| Lactococcins A, B, and M | 12 | 0.006 | |
| Nisin | 5 | 0.004 | |
| L. lactis MG1363acmAΔ1 | None | 0 | 0.001 |
| Bacteriocin of L. lactis IFPL105 | 0 | 0.001 | |
| Lactococcins A, B, and M | 2.5 | 0.002 | |
| Nisin | 0 | 0.001 |
a
Estimated by reduction of OD600.
b
1 U = 1 μmol of p-nitroaniline released/min/ml.
c
Cell suspensions in the supernatant fraction of an overnight culture of L. lactis MG1363acmAΔ1 (OD600 of 2) filtered through a 0.22-μm-pore-size filter, used as a control.