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. 2022 Jun 21;11:e78454. doi: 10.7554/eLife.78454

Figure 7. Adaptation to pellicle growth via increased expression of lpdA.

(A) Diagram of convergent adaptation in L4.4.1.1 strains MT540 and MT49 in the region upstream of lpdA within a transcription factor binding site (TFBS) and non-coding RNA. (B) Expression of lpdA differed significantly between sample groups (Kruskal–Wallis test, p-value=1.89e-04). Pairwise comparisons between groups (n=3, Mann-Whitney U test with Benjamini-Hochberg correction) revealed significantly increased expression in evolved (E) strains when compared with ancestral (A) strains, as well as during biofilm (B) growth when compared with planktonic (P) growth. (C) Overexpression of lpdA in H37Rv results in significantly (n=3 for each construct, Mann-Whitney U test) increased biofilm wet weights. p-value legend: *p<0.05, **p<0.01.

Figure 7—source data 1. RT-qPCR of lpdA (raw and log-2 fold changes) and pellicle wet weights from lpdA overexpression constructs shown in Figure 7B–C.

Figure 7.

Figure 7—figure supplement 1. H37Rv pellicles with empty vector (left) and extra copy of lpdA (right) introduced into the chromosome using pCT94.

Figure 7—figure supplement 1.

Qualitative pellicle phenotypes remain the same after introduction of additional lpdA.