REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-GIGYF2	Proteintech	Cat# 24790-1-AP; RRID:AB_2879727
Rabbit anti-DDX6	Proteintech	Cat# 14632-1-AP; RRID:AB_2091264
Rabbit anti-CNOT9	Proteintech	Cat# 22503-1-AP; RRID:AB_11232413
Rabbit anti-eIF4E2/4EHP	Proteintech	Cat# 12227-1-AP; RRID:AB_10642945
Mouse anti-GAPDH	Proteintech	Cat# 60004-1-Ig; RRID:AB_2107436
Rabbit anti-ZNF598	Thermo Fisher Scientific	Cat# 703,601; RRID:AB_2815335
Mouse anti-Flag M2	Sigma-Aldrich	Cat# F1804; RRID:AB_262044
Mouse anti-V5 tag	Thermo Fisher Scientific	Cat# R960-25; RRID:AB_2556564
Mouse anti-6xHis	Thermo Fisher Scientific	Cat# MA1-21315-HRP; RRID:AB_2536989
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488	Thermo Fisher Scientific	Cat# A-11008; RRID:AB_143165
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-11005; RRID:AB_2534073
Sheep anti-Mouse IgG - Horseradish Peroxidase	GE Healthcare	Cat# NA931; RRID:AB_772210
Goat anti-Rabbit IgG - Peroxidase	Sigma-Aldrich	Cat# A6154; RRID:AB_258284
Bacterial and virus strains
BL21 (DE3) Gold competent ells	Agilent	Cat# 230,132
BL21-CodonPlus (DE3) competent cells	Agilent	Cat# 230,245
DH5α competent cells	Thermo Fisher Scientific	Cat# 18,265,017
Chemicals, peptides, and recombinant proteins
Recombinant His6-NSP2	This paper	N/A
Recombinant His6-NSP21−115	This paper	N/A
Recombinant His6-NSP2107−212	This paper	N/A
Recombinant His6-NSP2204−350	This paper	N/A
Recombinant His6-NSP2G262/265V	This paper	N/A
Recombinant GIGYF2743−1,085	This paper	N/A
Recombinant 4EHP	This paper	N/A
Biotinylated isoxazole	Sigma-Aldrich	Cat# 900,572
Critical commercial assays
Duolink In Situ Red Kit	Sigma-Aldrich	Cat# DUO92101
Dual luciferase assay	Promega	Cat# E1960
Experimental models: Cell lines
HEK293T cells	Sigma-Aldrich	Cat# 12,022,001-1VL
Flp-In T-REx HEK293 cells	Thermo Fisher Scientific	Cat# R78007
4EHPKO Flp-In T-REx HEK293 cells	(Jafarnejad et al., 2018)	N/A
GIGYF2KO Flp-In T-REx HEK293 cells	This paper	N/A
Recombinant DNA
Plasmid: pFRT/TO/FLAG/HA-DEST TNRC6C	(Landthaler et al., 2008)	Addgene plasmid #19885
Plasmid: pcDNA5-FRT-TO-FH-NSP2	David Tollervey (Unpublished)	Addgene plasmid #157683
Plasmid: pcDNA5-FRT-TO-FH-NSP2 G262V/G265V	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP21−212	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP21−350	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP21−509	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP2107−638	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP2204−638	This paper	N/A
Plasmid: pcDNA5-FRT-TO-FH-NSP2340−638	This paper	N/A
Plasmid: pSpCas9(BB)-2A-Puro	(Ran et al., 2013)	Addgene plasmid #62988
Plasmid: pSpCas9(BB)-2A-Puro-Gigyf2-sgRNA	This paper	N/A
Plasmid: pET28-His6-ZZ	(Barbarin-Bocahu and Graille, 2022)	N/A
Plasmid: pET28-His6-ZZ-NSP2	This paper	N/A
Plasmid: pET28-His6-ZZ-NSP2 G262V/G265V	This paper	N/A
Plasmid: pET28-His6-ZZ-NSP21−115	This paper	N/A
Plasmid: pET28-His6-ZZ-NSP2107−212	This paper	N/A
Plasmid: pET28-His6-ZZ-NSP2204−350	This paper	N/A
Plasmid: pCI-Neo	Promega	Cat# E1841
Plasmid: pCI-Neo-V5-GIGYF2	This paper	N/A
Plasmid: pCI-Neo-V5-GIGYF21−267	This paper	N/A
Plasmid: pCI-Neo-V5-GIGYF2258−495	This paper	N/A
Plasmid: pCI-Neo-V5-GIGYF2486−752	This paper	N/A
Plasmid: pCI-Neo-V5-GIGYF2743−1,085	This paper	N/A
Plasmid: pCI-Neo-V5-GIGYF21,076-1,320	This paper	N/A
Plasmid: pCI-Neo-V5-4EHP	This paper	N/A
Plasmid: pCI-Neo-V5-4EHP W95A	This paper	N/A
Plasmid: pGEX-6P-1	Amersham	Cat# 27-4597-01
Plasmid: pGEX-6P-1-4EHP	This paper	N/A
Plasmid: pGEX-6P-1-GIGYF2743−1,085	This paper	N/A
Plasmid: pCI-Neo-λNV5-GIGYF2	This paper	N/A
Plasmid: pCI-Neo-λNV5-GW182SD	This paper	N/A
Plasmid: pCI-Neo-λNV5-GW182SD ΔPPGL	This paper	N/A
Plasmid: psiCHECK-2	Promega	Cat# C802A
Plasmid: psiCHECK-2-Ifnb1 3′ UTR	(Zhang et al., 2021)	N/A
Plasmid: pCI-Neo-RLuc-5BoxB-A114-N40-HhR	(Chapat et al., 2017b)	N/A
Plasmid: pCI-Neo-RLuc-5BoxB	(Pillai et al., 2004)	N/A
Plasmid: pCI-Neo-RLuc-6let7a	(Pillai et al., 2004)	N/A
Plasmid: pCI- Neo-FLuc	(Pillai et al., 2004)	N/A
Software and algorithms
Fiji	Fiji	RRID: SCR_002285
Prism 9	GraphPad	RRID:SCR_002798
Illustrator CS6	Adobe	RRID:SCR_010279
ChimeraX	UCSF	RRID:SCR_015872
Image Lab Software	Bio-Rad	RRID:SCR_014210
Jalview	Jalview	RRID:SCR_006459