REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-RIG-I, clone Alme-1	AdipoGen Life Sciences	Cat# AG-20B-0009; RRID: AB_2490189
Rabbit anti-MDA5, clone D74E4	Cell Signaling Technology	Cat# 5321; RRID: AB_10694490
Mouse anti-IRF1, clone H8	Santa Cruz Biotechnology	Cat# sc-74530; RRID: AB_2126826
Rabbit anti-IRF3, clone D6I4C	Cell Signaling Technology	Cat# 11904; RRID: AB_2722521
Rabbit anti-IRF5, clone E7F9W	Cell Signaling Technology	Cat# 76983; RRID: AB_2920569
Rabbit anti-IRF7	Proteintech	Cat# 22392-1-AP; RRID: AB_2879097
Mouse anti-IRF7, clone G-8	Santa Cruz Biotechnology	Cat# sc-74472; RRID: AB_2280489
Rabbit anti-Flag, clone D6W5B	Cell Signaling Technology	Cat# 14793; RRID: AB_2572291
Mouse anti-Flag, clone M2	Sigma-Aldrich	Cat# F3165; RRID: AB_259529
Rat anti-HA, clone 3F10	Roche	Cat# 11867423001; RRID: AB_390918
Mouse anti-GAPDH, clone 6C5	Sigma-Aldrich	Cat# MAB374; RRID: AB_2107445
Goat anti-mouse AF488	Thermo Fisher Scientific	Cat# A-11029; RRID: AB_2534088
Goat anti-rabbit AF488	Thermo Fisher Scientific	Cat# A-11034; RRID: AB_2576217
Goat anti-rat AF647	Thermo Fisher Scientific	Cat# A-21247; RRID: AB_141778
Mouse IgG HRP Linked Whole Ab	GE Healthcare	Cat# NA931; RRID: AB_772210
Rabbit IgG HRP Linked Whole Ab	GE Healthcare	Cat# NA934; RRID: AB_772206
Recombinant human anti-IFNAR2, clone REA124	Miltenyi Biotec	Cat# 130-099-555; RRID: AB_2652222
Bacterial and virus strains
H4 SeV	(Strahle et al., 2006)	N/A
IAV A/WSN/33 (H1N1)	Institut Pasteur, Paris, France	N/A
WNV-Tunisia-1997	Dr I. Leparc Goffart (French National Reference Center on Arboviruses, Marseille, France)	N/A
HIV-1 NL4-3 Luc	(He et al., 1995)	N/A
SARS-CoV-2 (BetaCoV/France/IDF0372/2020)	Institut Pasteur, Paris, France	N/A
SARS-CoV-2-mNeonGreen	(Xie et al., 2020)	N/A
Chemicals, peptides, and recombinant proteins
Gilteritinib	Chemietek	Cat# CT-GILT CAS: 1254053-43-4
BLZ-945	MedChemExpress	Cat# HY-12768 CAS: 953769-46-5
Nintedanib	MedChemExpress	Cat# HY-50904 CAS: 656247-17-5
PS-1145	Selleckchem	Cat# S7691 CAS: 431898-65-6
PD98059	MedChemExpress	Cat# HY-12028 CAS: 167869-21-8
RO-31-8425.HCl	MedChemExpress	Cat# HY-108136A CAS: 145317-11-9
Semaxinib	MedChemExpress	Cat# HY-10374 CAS: 204005-46-9
BX-795	MedChemExpress	Cat# HY-10514 CAS: 702675-74-9
Amlexanox	MedChemExpress	Cat# HY-B0713 CAS: 68302-57-8
U-0126-EtOH	MedChemExpress	Cat# HY-12031 CAS: 1173097-76-1
Rapamycin	MedChemExpress	Cat# HY-10219 CAS: 53123-88-9
SB-202190	MedChemExpress	Cat# HY-10295 CAS: 152121-30-7
BAY 11-7085	MedChemExpress	Cat# HY-10257 CAS: 196309-76-9
Wortmannin	MedChemExpress	Cat# HY-10197 CAS: 19545-26-7
Staurosporine	MedChemExpress	Cat# HY-15141 CAS: 62996-74-1
BAY 11-7082	MedChemExpress	Cat# HY-13453 CAS: 19542-67-7
AG 490	MedChemExpress	Cat# HY-12000 CAS: 133550-30-8
MRT67307	MedChemExpress	Cat# HY-13018 CAS: 1190378-57-4
BCI - 000209	BCI PHARMA	N/A
BCI - 001736	BCI PHARMA	N/A
BCI - 001775	BCI PHARMA	N/A
Ribavirin	Merck	Cat# R9644 CAS: 36791-04-5
Remdesivir	MedChemExpress	Cat# HY-104077 CAS: 1809249-37-3
Favipiravir	MedChemExpress	Cat# HY-14768 CAS: 259793-96-9
Hydroxychloroquine	Merck	Cat# H0915 CAS: 747-36-4
4-HPR	Merck	Cat# 390900 CAS: 65646-68-6
Scutellarein	MedChemExpress	Cat# HY-N0752 CAS: 529-53-3
GRL-0617	MedChemExpress	Cat# HY-117043 CAS: 1093070-16-6
Recombinant Human IFN-α2a	R&D Systems	Cat# 11100-1
Ivermectin	Merck	Cat# I8898 CAS: 70288-86-7
Critical commercial assays
CellTiter-Glo® Luminescent Cell Viability Assay	Promega	Cat# G7570
Nano-Glo® Luciferase Assay System	Promega	Cat# N1110
Nano-Glo® Live Cell Assay System	Promega	Cat# N2011
Experimental models: Cell lines
HeLa	ATCC	Cat# CCL-2
A549	ATCC	Cat# CCL-185
HEK293T/17	ATCC	Cat# CRL-11268
A549-ACE2	Olivier Schwartz (Institut Pasteur, Paris, France)	Derived from ATCC Cat# CCL-185
HEK-ACE2	Olivier Schwartz (Institut Pasteur, Paris, France)	Derived from ATCC Cat# CRL-11268
Vero	ATCC	Cat# CCL-81
Vero E6	Merck	Cat# 85020206
HCT116	ATCC	Cat# CCL-247
STING-37	(Lucas-Hourani et al., 2013)	N/A
C6/36	ATCC	Cat# CRL-1660
Oligonucleotides
ON-TARGETplus Human PPIB (5479) siRNA SMARTpool	Horizon Discovery	Cat# L-004606
ON-TARGETplus Human IRF3 (3661) siRNA SMARTpool	Horizon Discovery	Cat# L-006875
ON-TARGETplus Human IRF7 (3665) siRNA SMARTpool	Horizon Discovery	Cat# L-011810
ON-TARGETplus Human AXL (558) siRNA SMARTpool	Horizon Discovery	Cat# L-003104
ON-TARGETplus Human FLT3 (2322) siRNA SMARTpool	Horizon Discovery	Cat# L-003137
All qPCR primers are shown in Table S1		N/A
Recombinant DNA
pEFBOS(+)-Flag-2CARD	(Yoneyama et al., 2004)	N/A
pCenNLS-NLuc	This paper	N/A
pCenCBP-NLuc	This paper	N/A
pIRF1-α	This paper	N/A
pIRF3-α	This paper	N/A
pIRF3-α	This paper	N/A
pIRF7-α	This paper	N/A
Software and algorithms
Fiji	Open source, GNU General Public License.	N/A
FlowJo	Treestar Inc.	N/A
FACSDiva	Becton Dickinson	N/A
GraphPad Prism	GraphPad Software	N/A
Other
Hoechst 33342	ThermoFisher Scientific	Cat# H3570
Fluoromount™ Aqueous Mounting Medium	Merck	Cat# F4680
Basticidin	Invivogen	Cat# ant-bl-05
Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate)	ThermoFisher Scientific	Cat# 11811098
FuGENE® 6 Transfection Reagent	Promega	Cat# E2691
HiPerFect Transfection Reagent	Qiagen	Cat# 301705
Bright-Glo™ Luciferase Assay System	Promega	Cat# E2610