FIG. 4.

Relative molecular weight of the proteins used in this study. A Sephacryl S200 HR column was calibrated with protein standards of known molecular weight. The standard curve was plotted with the molecular weight (in thousands) versus the V_e/V_o ratio. V_e is the elution volume and V_o is the void volume as determined by blue dextran. DehIVa, HKU221, HKU272, HKU273, HKU275, HKU281, HKU285, and HKU356 are dimers and eluted around 46K (within the open circle). DehCI, HKU252, HKU271, HKU284, HKU357, and HKU359 are monomers and eluted around 30K (within the open square). HKU362 and HKU363 contain both dimer and monomer and were found eluted around the 46K and the 30K regions.