TABLE 1.

Primers used for generation of mutations in the hdlIVa gene

Primer sequencesa	Mutation	Construct
5′-G-ATG-AAT(CAT)-CAG-TAT-GCG-G-3′	H56N	pHKU356
5′-G-ATG-CAT-CAG-TTT(TAT)-GCG-G-3′	Y58F	pHKU357
5′-AT-GCG-GAT-TAT(TTT)-TGG-C-3′	F61Y	pHKU358
5′-GG-CAG-TTG-GCC(ACC)-GAT-G-3′	T65A	pHKU359
5′-G-ACC-GAT-GAG-ACG(GCG)-CTG-AC-3′	A68T	pHKU360
5′-G-ACC-GAT-GAG-GCG-ATG(CTG)-AC-3′	L69M	pHKU361
5′-AC-CAT-ATG(CTC)-GAG-GAT-CGC-3′	L78M	pHKU362
5′-G-ATG-AGT-GCG-TTC(TAC)-AAG-G-3′	Y92F	pHKU363
5′-C-GAA-AAG-ATG(TTG)-AAA-TC-3′	L108M	pHKU364

^aThe mutated nucleotide(s) is shown in boldface type, and the wild-type sequence is shown in parentheses.