TABLE 2.
Dehalogenase activities of CE for DehIVa-, DehCI-, HKU284-, and HKU285-producing strains
| Substrate | Sp act (μM halide released/mg/min)a
|
|||
|---|---|---|---|---|
| DehIVa | DehCI | HKU284b | HKU285c | |
| MCA | 24.7 (1) | 53.3 (1) | 28.0 (1) | 32.7 (1) |
| MBA | 34.0 (1.4) | 42.8 (0.8) | 24.0 (0.36) | 27.4 (0.8) |
| L-2MCPA | 5.9 (0.24) | 9.9 (0.19) | 4.1 (0.15) | 7.7 (0.24) |
| DL-2MBPA | 10.3 (0.42) | 16.1 (0.30) | 9.5 (0.34) | 9.7 (0.30) |
| DCA | — | 4.1 (0.08) | — | 4.2 (0.13) |
a
Activities are averages of two experiments that show no significant deviation. —, no detectable activity. The relative activity with respect to MCA as the 100% was shown in parentheses.
b
HKU284 is DehIVa1–73–DehCI73–89–DehIVa91–231.
c
HKU285 is DehCI1–72–DehIVa74–90–DehCI90–227.