Figure 2.

Loss of tissue integrity assessment with confocal Brillouin microscopy. (a) Schematic representation of proximal RZ of the distal phalanx in digit II, generated with Keynote. (b) Widefield fluorescence images of region in CAGGs:GFP hands treated with collagenase at different times, which were probed using confocal Brillouin microscopy. (i) No treatment, (ii) 30 min, (iii) 45 min. Scale bar: 50 µm. (c) Brillouin elastic contrast ($\overline{{\nu }_{\mathrm{B}}}$) maps for the regions shown in (b). (i) No treatment, (ii) 30 min, (iii) 45 min. Maps reveal a time-dependent loss of tissue integrity upon collagenase treatment. (d) Average $\overline{{\nu }_{\mathrm{B}}}$ values of maps generated from CAGGs:GFP hands treated with collagenase. N_A = 5, Friedman ANOVA with Dunn's post hoc analysis, **p < 0.01.