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. 2022 Jun 14;2022:7216758. doi: 10.1155/2022/7216758

Figure 2.

Figure 2

Production of exogenous HER2-miR1 mature form from its precursor. (a) Fluorescent image (×40) of HEK293t cells transfected with mock vector or the expression vector that carries HER2-miR1 precursor. (b) RT-qPCR results indicated the overexpression of HER2-miR1 (~×4,000 folds) in the HEK293t cells that were transfected in (a). Data were normalized against U48, and error bars indicate standard deviation (SD) of duplicated experiments. (c) Electrophoresis gel image demonstrated the appropriate size of mature HER2-miR1 in the cells overexpressing preHER2-mir1 in comparison with un-transfected cells. (d) Sequencing result of two TA vector clones (named clones 1 and 2) containing HER2-miR1, which is aligned with preHER2-mir1. 3′-end of HER2-miR1 was at least three nucleotides longer than the specific primer shown by a black arrow which was used for amplification of this miRNA. The downstream and upstream nucleotides to HER2-miR1 sequence belong to the vector and anchored oligo-dT primer.