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. 2022 Jun 3;7(3):982–988. doi: 10.1016/j.synbio.2022.05.008

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Verification of the feasibility of the regulation module and the response module of the CRISPRi-mediated self-inducible system (CiMS). (A) Construction of the itaconic acid (ITA) biosensor YpItcR/P_ccl. (B) Analysis of the RFP fluorescence intensity to verify the expression strength of YpItcR/P_ccl with different concentrations of ITA. (C) Construction of the regulative module and response module of the CiMS. (D) Analysis of the RFP fluorescence intensity to verify the efficiency of interference by the CiMS. Error bars show the standard deviation from three independent experiments.